1. Fig. 1. Identification of SE-associated lncRNAs.
Identification of SE-associated lncRNAs. (A) Selection strategy of novel SE-associated lncRNAs from a genome-wide profiling of the cardiac transcriptome after MI. (B) Schematic of an intergenic lncRNA located between two PCGs. Heat map showing clustering of heart-enriched intergenic lncRNAs differentially expressed after MI (adjusted P < 0.01). (C) Volcano plot of lncRNAs associated with TEs (triangle) or with SEs (circle). The x axis shows lncRNAs expression in infarcted versus sham-operated animals (log2 fold change) quantified from RNA-seq data; the y axis shows adjusted P value. Wisper expression in sham-operated (black bar) and infarcted heart (red bar) is expressed in FPKM (fragments per kilobase of exon per million fragments mapped). Bars represent mean ± SEM (n = 4). P value was determined by Student’s t test. (D) qRT-PCR analysis of SE-associated lncRNAs (SE-lncRNA) and PCG expression in CMs and fibroblasts isolated from neonatal mouse hearts. Data represent fold change ratio (CM/CF) mean ± SEM (n = 3). P value was determined by Student’s t test. (E) H3K27Ac signature of the locus encompassing Wisper in different human tissues. The red bar highlights the SE region.
Rudi Micheletti et al., Sci Transl Med 2017;9:eaai9118
Published by AAAS