1. Volume 139, Issue 4, Pages 1081-1083.e5 (October 2010)
Epithelial to Mesenchymal Transition in Injury of Solid Organs: Fact or Artifact? 
Jeremy S. Duffield 
Gastroenterology 
Volume 139, Issue 4, Pages e5 (October 2010)
DOI: /j.gastro
Copyright © 2010 AGA Institute Terms and Conditions

2. Supplemental Figure 1
Fate mapping of hepatocytes in advanced liver fibrosis. (A) Schema of potential sources of myofibroblasts in the hepatic lobule based on in vitro observations. The hepatic lobule comprises specialized capillaries called hepatic sinusoids, lined by endothelial cells and HSCs. Alongside these are hepatocytes that are polarized and secrete both into the space of Disse and the bile duct cannaliculi. Monocytes and myeloid derived Kupffer cells are also present. All of these cells in health have been implicated by in vitro studies as potential sources of myofibroblasts. (B) Schematic showing lineage tracing strategy for mice transgenic for Alb-Cre and the reporter Z/Red. When mice are crossed, loxP sites are recombined in albumin-producing cells removing a stop sequence from genomic DNA and activating dsRED (RFP) under the β-actin/CMV promoter enhancer. (C) Confocal images showing sections of mouse liver labeled with DAPI to identify nuclei, 12 weeks after biweekly systemic CCl4 administration (CCl4). Note in negative control Z/Red diseased mice that no hepatocytes are labeled with the fate marker RFP, but there are autofluorescent cells around and within scars (arrows). By contrast, in Alb-Cre;Z/Red mice many hepatocytes can be detected with the fate marker RFP in cytoplasm and nucleus, but cells in scars do not have the RFP fate marker. (D) Confocal images showing sections of mouse liver immunolabeled with antibodies against Pdgfrβ (green) 12 weeks after biweekly systemic CCl4 administration (CCl4) or vehicle only (Control). Note in Alb-Cre;Z/Red mice after accounting for autofluorescent cells (arrows), no RFP can be seen clearly in nuclei and cytoplasm in the scars or scar associated myofibroblasts detected by Pdgfrβ. In liver scars of negative control Z/Red mice (where no recombination has occurred and no RFP is activated) and also Alb-Cre;Z/Red mice there is significant autofluorescent material, particularly in scar associated macrophages (arrows), which appear yellowish in merged images. In positive control NLS-Cre;Z/Red mice where recombination and activation of RFP occurs in all cells, hepatic stellate cells (identified by Pdgfrβ expression) co-express the fate marker RFP indicating that if myofibroblasts had derived from hepatocytes in Alb-Cre;Z/Red mice RFP would be detected (marker = 50 μm).
Gastroenterology  , e5DOI: ( /j.gastro )
Copyright © 2010 AGA Institute Terms and Conditions