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Mechanism of neutrophil recruitment induced by IL-8 in chronic sinusitis  Hideaki Suzuki, MDa, Yuichi Takahashi, MDb, Hideya Wataya, MDa,c, Katsuhisa Ikeda,

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Presentation on theme: "Mechanism of neutrophil recruitment induced by IL-8 in chronic sinusitis  Hideaki Suzuki, MDa, Yuichi Takahashi, MDb, Hideya Wataya, MDa,c, Katsuhisa Ikeda,"— Presentation transcript:

1 Mechanism of neutrophil recruitment induced by IL-8 in chronic sinusitis 
Hideaki Suzuki, MDa, Yuichi Takahashi, MDb, Hideya Wataya, MDa,c, Katsuhisa Ikeda, MDa, Seiichiro Nakabayashi, MDa, Akira Shimomura, MDa, Tomonori Takasaka, MDa  Journal of Allergy and Clinical Immunology  Volume 98, Issue 3, Pages (September 1996) DOI: /S (96) Copyright © 1996 Mosby, Inc. Terms and Conditions

2 FIG. 1 Profile of infiltrated polymorphonuclear cells in patients with chronic sinusitis and patients with allergic rhinitis. Each value represents the ratio of neutrophils and eosinophils to the total number of polymorphonuclear cells (n = 10). Bars indicate mean ± SEM. **p < Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

3 FIG. 2 Immunohistochemical staining for IL-8 of the nasal smear. A, Chronic sinusitis. Positive staining is observed in the cytoplasm of polymorphonuclear cells. B, Allergic rhinitis. C, Chronic sinusitis. Negative control. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

4 FIG. 2 Immunohistochemical staining for IL-8 of the nasal smear. A, Chronic sinusitis. Positive staining is observed in the cytoplasm of polymorphonuclear cells. B, Allergic rhinitis. C, Chronic sinusitis. Negative control. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

5 FIG. 2 Immunohistochemical staining for IL-8 of the nasal smear. A, Chronic sinusitis. Positive staining is observed in the cytoplasm of polymorphonuclear cells. B, Allergic rhinitis. C, Chronic sinusitis. Negative control. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

6 FIG. 3 Immunohistochemical staining for IL-8 of mucosal sections. A, Chronic sinusitis. Nasal gland duct cells are positively stained. B, Chronic sinusitis. Mucus in the nasal gland shows strong reactivity. C, Allergic rhinitis. D, Chronic sinusitis. Negative control. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

7 FIG. 3 Immunohistochemical staining for IL-8 of mucosal sections. A, Chronic sinusitis. Nasal gland duct cells are positively stained. B, Chronic sinusitis. Mucus in the nasal gland shows strong reactivity. C, Allergic rhinitis. D, Chronic sinusitis. Negative control. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

8 FIG. 3 Immunohistochemical staining for IL-8 of mucosal sections. A, Chronic sinusitis. Nasal gland duct cells are positively stained. B, Chronic sinusitis. Mucus in the nasal gland shows strong reactivity. C, Allergic rhinitis. D, Chronic sinusitis. Negative control. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

9 FIG. 3 Immunohistochemical staining for IL-8 of mucosal sections. A, Chronic sinusitis. Nasal gland duct cells are positively stained. B, Chronic sinusitis. Mucus in the nasal gland shows strong reactivity. C, Allergic rhinitis. D, Chronic sinusitis. Negative control. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

10 FIG. 4 In situ hybridization for IL-8 mRNA of mucosal sections. A, Chronic sinusitis. Expression of mRNA can be seen in nasal gland duct cells. B, Allergic rhinitis. C, Chronic sinusitis. Negative control. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

11 FIG. 4 In situ hybridization for IL-8 mRNA of mucosal sections. A, Chronic sinusitis. Expression of mRNA can be seen in nasal gland duct cells. B, Allergic rhinitis. C, Chronic sinusitis. Negative control. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

12 FIG. 4 In situ hybridization for IL-8 mRNA of mucosal sections. A, Chronic sinusitis. Expression of mRNA can be seen in nasal gland duct cells. B, Allergic rhinitis. C, Chronic sinusitis. Negative control. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

13 FIG. 5 In situ hybridization for IL-8 mRNA of the nasal smear. A, Chronic sinusitis. Polymorphonuclear cells exhibit positive staining. B, Allergic rhinitis. C, Chronic sinusitis. Negative control. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

14 FIG. 5 In situ hybridization for IL-8 mRNA of the nasal smear. A, Chronic sinusitis. Polymorphonuclear cells exhibit positive staining. B, Allergic rhinitis. C, Chronic sinusitis. Negative control. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

15 FIG. 5 In situ hybridization for IL-8 mRNA of the nasal smear. A, Chronic sinusitis. Polymorphonuclear cells exhibit positive staining. B, Allergic rhinitis. C, Chronic sinusitis. Negative control. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

16 FIG. 6 IL-8 level in nasal discharge. Nasal discharge was collected by suction, diluted 5 to 10 times with saline solution and centrifuged at 1000 g for 5 minutes. The supernatant was then subjected to enzyme immunoassay for IL-8 with Biotrak IL-8 ELISA system (Amersham International plc.) according to the manufacturer’s instructions. *p < Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

17 FIG. 7 Hypothesis of neutrophil recruitment in chronic sinusitis. Neutrophil chemotactic substances including IL-8 released from nasal gland duct cells and epithelial cells (thin solid arrow) initiate neutrophil migration (striped arrows) out of vessels to the lumenal side and eventually into sinus effusion (wavy arrows). Emigrated neutrophils produce and secrete IL-8 (thick solid arrow), which further induces neutrophil migration and accumulation in sinus effusion. Bacteria and bacterial components may stimulate neutrophils, epithelial cells, and nasal gland duct cells to secrete IL-8 (dotted arrows). Accumulated neutrophils in sinus effusion probably release proteases and superoxides, which impair mucociliary function of the paranasal epithelium, and this causes retention of sinus effusion. As a result, the whole inflammatory process is prolonged. LTB 4, Leukotriene B 4; fMLP, N-formyl-methionyl-leucyl-phenylalanine. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

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