1. Volume 6, Issue 3, Pages 743-756 (May 2013)
OsMADS16 Genetically Interacts with OsMADS3 and OsMADS58 in Specifying Floral Patterning in Rice 
Dapeng Yun, Wanqi Liang, Ludovico Dreni, Changsong Yin, Zhigang Zhou, Martin M. Kater, Dabing Zhang 
Molecular Plant 
Volume 6, Issue 3, Pages (May 2013)
DOI: /mp/sst003
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

2. Figure 1
Double In Situ Analysis of OsMADS3 and OsMADS16 in the Wild-type Flowers.
(A–C) OsMADS16 expression in the wild-type flower at early stage.
(D–F) Transcripts of OsMADS3 in the same histological sections above. (A) and (D) Inflorescence primordium at stage In7, and the developmental stages of inflorescence and flower defined by Ikeda et al. (2004). (B) and (E) Early-stage SP4; (C) and (F) later-stage SP4.
(G–J) Double color-reaction of OsMADS3 and OsMADS16 in wild-type flower primordium. Arrow in (G) indicates the second-whorl founder cells expressing only OsMADS16 as indicated in (C), (F), and (H). Purple: OsMADS3; Yellow: OsMADS16; Brown: overlap of OsMADS3 and OsMADS16. le, lemma; lo, lodicule; pa, palea; pb, primary branch; sb, secondary branch; sp, spikelet; st, stamen; fm, floral meristem. Bars = 50 μm.
Molecular Plant 2013 6, DOI: ( /mp/sst003)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

3. Figure 2. Flower Phenotype of spw1-1 osmads3-4 and spw1-1 osmads58.
AWild-type (WT) flower after the removal of the lemma/palea.
BA spw1-1 flower with glume- and carpeloid glume-like organs, and ectopic carpels.
CAn osmads3-4 mutant flower. Arrowheads and arrows indicate normal stamens and lodicule–stamen mosaic organs, respectively.
DAn osmads58 mutant flower showing similar phenotype to WT flower.
EA spw1-1 osmads3-4 double mutant showing multiple glume-like organs in whorl 2 (white arrowheads) and whorl 3 (yellow arrowheads) adjacent to the ectopic carpels.
FTransverse section of a spw1-1 osmads3-4 spikelet at the position indicated in (E) by the red arrow, showing three glume-like organs in whorl 2 (white arrowheads) and six glume-like organs in whorl 3 (yellow arrowheads) adjacent to the ectopic carpels (black arrows).
GTransverse section of a spw1-1 osmads3-4 spikelet at a lower position.
HA spw1-1 osmads58 double mutant flower showing glume-like organs in whorl 2 (white arrowheads) and in whorl 3 (yellow arrowheads), where they are adjacent to the ectopic carpels.
ITransverse section of a spw1-1 osmads58 spikelet at the position indicated in (H) by the red arrow, showing two glume-like organs (white arrowheads) in whorl 2, three glume-like organs/carpeloid glume-like organs in whorl 3 (yellow arrowheads), and carpel-like organs (black arrows).
JClose-ups of the marginal region of palea (mrp) indicated in (F) and two types of cells: spongy parenchymatous cell (spc) and nonsilicified cell (nsc).
(K, L) Close-ups of the glume-like organ (K) indicated in (F) and the glume-like organ (L) indicated in (I) to show the same cell types with mrp. Spongy parenchymatous cell (spc) and nonsilicified cell (nsc).
MTransverse section of a wild-type spikelet in a basal position.
(N–S) Floral diagrams of wild-type (N), spw1-1 (O), osmads3-4 (P), osmads58 (Q), spw1-1 osmads3-4 (R), spw1-1 osmads3-4 (S). cgl, carpeloid glume-like organ; ec, ectopic carpel; eg, empty glume; le, lemma; lo, lodicule; pa, palea; pi, pistil; gl, glume-like structure; st, stamen. Bars = 1 mm in (A)–(E), (G), and (L); 100 μm in (F), (H)–(K), and (M).
Molecular Plant 2013 6, DOI: ( /mp/sst003)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

4. Figure 3.
Scanning Electron Microscopy (SEM) Analysis of Flowers at Early Stages.
(A–D) SEM images of wild-type flowers at stages Sp4 (A), Sp6 (B), Sp7 (C), and Sp8 (D).
Arrowheads in (B) indicate lodicule primordia and the arrow in (C) indicates a carpel primordium. Asterisks in (B)–(D) indicate stamen primordia or stamens.
(E–J) Early development of the spw1-1 osmads3-4 flower.
(K, L) Early development of the spw1-1 osmads58 flower.
(M–O) Early development of the spw1-1 flower. Arrows in (H) indicate carpel primordia. White and yellow arrowheads in (H)–(J), (L), (M), and (O) indicate glume-like organs in whorls 2 and 3, respectively. ca, carpel; ec, ectopic carpel; fm, floral meristem; le, lemma; pa, palea; st, stamen.?: primordia of glume-like organs or ectopic carpels. Bars = 50 µm in (A)–(G) and (E)–(O), 200 µm in (D).
Molecular Plant 2013 6, DOI: ( /mp/sst003)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

5. Figure 4.
Scanning Electron Microscopy (SEM) Analysis of spw1-1 osmads3-4 and spw1-1 osmads58.
(A, B) The flower of spw1-1 osmads3-4 (A) and spw1-1 osmads58 (B). White arrowheads indicate glume-like organs in whorl 2.
(C, D) SEM of the abaxial surface of glume-like organs of spw1-1 osmads3-4 (C) and spw1-1 osmads58 (D).
ESEM of the abaxial, marginal region of the palea in wild-type indicated in Figure 3D.
(F, G) SEM of the abaxial surface of glume-like organs of spw1-1 osmads3-4 (F) indicated in (C) and spw1-1 osmads58 (G) indicated in (D), respectively.
HSEM of the adaxial, marginal region of the palea in the wild-type.
(I, J) SEM of the adaxial surface of glume-like organs of spw1-1 osmads3-4 (I) and spw1-1 osmads58 (J), respectively. ec, ectopic carpel; Bars = 1 mm in (A) and (B), 100 µm in (C) and (D), 10 µm in (E)–(J).
Molecular Plant 2013 6, DOI: ( /mp/sst003)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

6. Figure 5.
In Situ Analysis of the Expression Pattern of OsMADS1, OsMADS6, and DL in Wild-Type and Double Mutants.
(A–C) OsMADS1 transcripts in the wild-type at stages Sp4, Sp5, and Sp7.
(D–G) OsMADS1 transcripts in the spw1-1 osmads3-4 (D)–(F) and spw1-1 mads58 (G).
(H–J) OsMADS6 transcripts in the wild-type at stages Sp4, Sp6, and Sp7.
(K–N) OsMADS6 transcripts in the spw1-1 osmads3-4 (K)–(M) and spw1-1 osmads58 (N).
(O, P) DL transcripts in the wild-type at stage In6 and at stage Sp8.
(Q–U) DL transcripts in the spw1-1 osmads3-4 (Q)–(T) and spw1-1 osmads58 (U). Arrowheads and arrows indicate glume-like organs and ectopic carpels, respectively. The blue arrowhead in (U) indicates carpeloid glume-like organ. ca, carpel; ec, ectopic carpel; fm, floral meristem; le, lemma; lo, lodicule; pa, palea; pl, palea-like structure; st, stamen. Bars = 50 µm in (A), (B), (D), (E), (H), and (K); 100 µm in (C), (F), (G), (I), (J), and (L)–(U).
Molecular Plant 2013 6, DOI: ( /mp/sst003)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

7. Figure 6.
In Situ Analysis of the Expression Pattern of OsMADS16, OsMADS3, and OSMADS58.
(A, B) OsMADS16 transcripts in the wild-type at stages Sp5 and Sp7.
(C, D) OsMADS16 transcripts in the osmads3-4 mutant at stages Sp5 and Sp6.
(E, F) OsMADS16 transcripts in the osmads58 mutant at stages Sp5 and Sp6.
(G, H) OsMADS3 transcripts in the spw1-1 mutant.
(I, J) OsMADS58 transcripts in the wild-type at stages Sp6 and Sp8.
(K, L) OsMADS58 transcripts in the spw1-1 mutant. Arrow in (H) indicates glume-like organs. ca, carpel; ec, ectopic carpel; fm, floral meristem; le, lemma; lo, lodicule; ov, ovule; pa, palea; st, stamen. Bars = 50 µm in (A)–(G), (J), and (L); 100 µm in (H), (I), and (K).
Molecular Plant 2013 6, DOI: ( /mp/sst003)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions