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Published byTrevon Sommers Modified over 10 years ago
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Details of Tune and Method Setup for LTQ Orbitrap
Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK
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Tune file - nanospray
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Tune file - nanospray
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Tune file - nanospray
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Tune file - nanospray
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Tune file - nanospray
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Tune file - nanospray
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Tune file - nanospray
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Tune file - nanospray
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Micro AS autosampler settings
Note: Micro AS must be put back to ‘Menu/Serial’ (use the touch screen) Note: in sample table the vial position should be ‘1a1’
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Micro AS autosampler settings
Wash solution: water
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Micro AS autosampler settings
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Surveyor LC settings
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Surveyor LC settings
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LTQ Orbitrap settings
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LTQ Orbitrap settings
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LTQ Orbitrap settings
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LTQ Orbitrap settings Exclusion duration set to about ½ peak width (here 15 sec)
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LTQ Orbitrap settings
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LTQ Orbitrap settings
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LTQ Orbitrap settings
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LTQ Orbitrap settings
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LTQ Orbitrap settings Scan event 2 – MS/MS of the 1st most intense ion
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LTQ Orbitrap settings Scan event 3 – MS/MS of the 2nd most intense ion
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LTQ Orbitrap settings Scan event 4 – MS/MS of the 3rd most intense ion
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Divert Valve
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Method Summary
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Method Summary
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