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Messenger ribonucleic acid for the gonadal luteinizing hormone/human chorionic gonadotropin receptor is not present in human endometrium  Elizabeth A.

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Presentation on theme: "Messenger ribonucleic acid for the gonadal luteinizing hormone/human chorionic gonadotropin receptor is not present in human endometrium  Elizabeth A."— Presentation transcript:

1 Messenger ribonucleic acid for the gonadal luteinizing hormone/human chorionic gonadotropin receptor is not present in human endometrium  Elizabeth A Stewart, M.D., Marine Sahakian, M.D., Alan Rhoades, B.A., Bradley J Van Voorhis, M.D., Romana A Nowak, Ph.D.  Fertility and Sterility  Volume 71, Issue 2, Pages (February 1999) DOI: /S (98)

2 FIGURE 1 Schematic diagram of the human LH/hCG receptor and the primer set used to amplify the cDNA for the receptor. This receptor has the classic structure of a seven-transmembrane–spanning, G-coupled receptor. The cell membrane is shown as a lipid bilayer. All primer sets amplify the extracellular region, except for the xantho primer pair, which amplifies transmembrane segments 2–6 wholly within exon 11. Fertility and Sterility  , DOI: ( /S (98) )

3 FIGURE 2 Polymerase chain reaction products visualized on a 1% agarose gel after two rounds of amplification with the LH primer set. A 647-bp fragment is seen in the lane containing granulosa cell cDNA (GC); no bands are seen in the lanes containing yeast cDNA (Y) as a negative control or in the five separate endometrial samples (E1-E5). The darkest band of the 100-bp ladder (LAD) represents the 800-bp fragment and is indicated by an arrow. The other 20 endometrial samples amplified produced no bands. Fertility and Sterility  , DOI: ( /S (98) )

4 FIGURE 3 Polymerase chain reaction products visualized on a 1% agarose gel after amplification with the xantho primer set. A 524-bp fragment is seen in the lane containing granulosa cell cDNA (GC), and no bands are seen in the lane containing yeast cDNA (Y) as a negative control. Bands are seen in four of the five endometrial samples (all except E3). A 100-bp ladder (LAD) is seen, with the darkest band representing an 800-bp fragment and indicated by an arrow. Bands were seen in 15 of the remaining 20 endometrial samples amplified. Sequencing confirmed the identity of the products. Fertility and Sterility  , DOI: ( /S (98) )


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