1. Effect of 5-aza-2′-deoxycytidine on methylation of the putative imprinted control region of H19 during the in vitro development of vitrified bovine two-cell embryos 
Xue-Ming Zhao, Ph.D., Jing-Jing Ren, M.Sc., Wei-Hua Du, Ph.D., Hai-Sheng Hao, Ph.D., Dong Wang, Ph.D., Yan Liu, Ph.D., Tong Qin, Ph.D., Hua-Bin Zhu, Ph.D. 
Fertility and Sterility 
Volume 98, Issue 1, Pages (July 2012)
DOI: /j.fertnstert
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
(A) Bisulfate sequencing results for a representative sample of blastocysts derived from vitrified two-cell embryos of the 5-aza-2′-deoxycytidine (5-aza-dC) group. Each line represents a unique DNA clone; filled and open circles represent methylated and unmethylated CpGs, respectively. (B) The percentage of methylated H19 putative imprinted control region clones in two-cell embryos and blastocysts. Values with different superscripts of the each type of embryos differ significantly between different groups. (C) The relative expression of H19 messenger RNA in blastocysts. Values with different superscripts differ significantly between different groups.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions