1. DNA degradation is strictly controlled in wild-type, but not in recA cells.
DNA degradation is strictly controlled in wild-type, but not in recA cells. A fraction of anucleate cells was determined in wild-type (AB1157) and recA populations, which were either unirradiated (left side of the panel) or irradiated with 400 Gy of γ-rays and then incubated for 90 min at 37° (right side of the panel). Cells were fixed with osmium tetroxide, their DNA stained with DAPI, and visualized by fluorescence microscopy. An anucleated cell count in an analyzed population from three experiments is represented in a table. DAPI, 4’,6-diamidino-2-phenylindole; wt, wild-type.
Edyta Đermić et al. G3 2017;7:
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