1. Quiz#2 LC710 10/15/12 name____________
Q1(8 %) You have identified this protein conserved across 4 species (non-rodent).
MNGTYEMRATLSMLWAAYMFLLIML *
MQGTYEMRASLTMLWAAYMFLLVML *
MQGTYEMRASLSMIWAAYMFLLIML *
MQGTYEMKASLSMLWAAYMFLLIML *
* = STOP
You make an antibody to this protein and find that it is found only in the eyes of all 4 species
1 2
1 2
1 2
1 2
You eventually want to identify this gene in mouse. How will you start? Using
_______________________as a PCR
template (2%) ?
Design 5’ to 3’ oligos that will give you the best chance at identifying some of
this coding sequence in the mouse.
You are fearful of getting to much garbage from your amplification!
Codon
Probability
In fish 1
In mouse 2
organisms
(1%)
Oligo1:5’_______________________________
(1%)
Oligo2: 5’_______________________________
(4%) How will you know that
Your PCR succeeded in amplifying
the correct fragment (be specific)?
_____________________________________
_____________________________________
_____________________________________