1. Olfactomedin-4 is a glycoprotein secreted into mucus in active IBD
Michael Gersemann, Svetlana Becker, Sabine Nuding, Lena Antoni, German Ott, Peter Fritz, Naohide Oue, Wataru Yasui, Jan Wehkamp, Eduard F. Stange 
Journal of Crohn's and Colitis 
Volume 6, Issue 4, Pages (May 2012)
DOI: /j.crohns
Copyright © 2011 European Crohn's and Colitis Organisation Terms and Conditions

2. Figure 1
IL-8, OLFM4, Muc1 and Hath1 expression in controls and IBD mucosa: IL-8 transcripts are enhanced in inflamed vs. noninflamed CD and UC without significant differences between both diseases (A). OLFM4 mRNA was significantly more induced in UC than CD (B). Muc1 transcripts are augmented more in inflamed vs. noninflamed CD than in UC (C). Hath1 mRNA is significantly downregulated in inflamed UC (D). OLFM4 is highly correlated with IL-8 (E, r=0.68 for all samples, p<0.001) but not with Hath1 (F, r=−0.17 for all samples, ns; squares = controls, triangles = CD, circles = UC; *: p<0.05 (these significances are lost after Bonferroni correction), **: p<0.01, ***: p<0.001).
Journal of Crohn's and Colitis 2012 6, DOI: ( /j.crohns )
Copyright © 2011 European Crohn's and Colitis Organisation Terms and Conditions

3. Figure 2
OLFM4 immunostaining in controls and IBD mucosa: OLFM4 staining is located in the lower third of the crypt in control samples (A, magnification 100-fold) and expanded up to the epithelial surface during inflammation (B = inflamed CD, C = inflamed UC, magnification 100-fold).
Journal of Crohn's and Colitis 2012 6, DOI: ( /j.crohns )
Copyright © 2011 European Crohn's and Colitis Organisation Terms and Conditions

4. Figure 3
OLFM4 Dot Blot analysis in controls and IBD mucosa: OLFM4 protein is significantly increased in inflamed CD and even more pronounced in inflamed UC biopsy samples as compared to controls (top lane: recombinant OLFM4 in increasing amounts from left to right: 0.05, 0.1, 0.5 and 1μg).
Journal of Crohn's and Colitis 2012 6, DOI: ( /j.crohns )
Copyright © 2011 European Crohn's and Colitis Organisation Terms and Conditions

5. Figure 4
Secreted OLFM4 in controls and IBD mucus: OLFM4 is found in the crypt lumen (A = CD, arrows, magnification 200-fold) and also in the surface mucus (B = UC, arrows, magnification 200-fold). Dot Blot analysis demonstrates OLFM4 in rectal mucus extracts (C, top lane: recombinant OLFM4 in increasing concentrations from left to right: 0.05, 0.1 and 0.5μg).
Journal of Crohn's and Colitis 2012 6, DOI: ( /j.crohns )
Copyright © 2011 European Crohn's and Colitis Organisation Terms and Conditions

6. Figure 5
OLFM4 binding to HBD1-3: Coincubation of OLFM4 with HBD1-3, but not bovine serum albumin (BSA), resulted in a dose dependent increase of OLFM4 absorption (*: p<0.05, ***: p<0.001).
Journal of Crohn's and Colitis 2012 6, DOI: ( /j.crohns )
Copyright © 2011 European Crohn's and Colitis Organisation Terms and Conditions

7. Figure 6
OLFM4 expression in LS174T cells incubated with E. coli Nissle (EcN) in the presence and absence of the γ-secretase inhibitor dibenzazepine (DBZ). 24h of incubation with EcN led to a significant increase of OLFM4 transcripts. Treatment with DBZ led to a significant downregulation of OLFM4 transcripts after 24h. DBZ blocked the stimulation by EcN. Values are compared to controls at the given time-point set to 1 (*: p<0.05, **: p<0.01). This experiment was performed for 4 times in triplicates.
Journal of Crohn's and Colitis 2012 6, DOI: ( /j.crohns )
Copyright © 2011 European Crohn's and Colitis Organisation Terms and Conditions