1. Molecular Stenting with a Crosslinked Hyaluronan Derivative Inhibits Collagen Gel Contraction 
Tanuj D. Mehra, Kaustabh Ghosh, Xiao Zheng Shu, Glenn D. Prestwich, Richard A.F. Clark 
Journal of Investigative Dermatology 
Volume 126, Issue 10, Pages (October 2006)
DOI: /sj.jid
Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Dose response of PDGF on fibroblast-mediated collagen gel contraction.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Effect of different forms of HA on collagen gel contraction. Contraction of the collagen gels is expressed as a difference in initial area (A0) at t=0 and final area (A1) at t=48hours. Contraction indices for (a) low MW H were 0.35 and 0 for 0.12 and 0.25% HA (w/v) respectively; (b) HMW HA were 2.04 and 3.56 for 0.12 and 0.25% HA (w/v) respectively; and (c) HA-DTPH were −2.32 and −10.61 for 0.12 and 0.25% HA (w/v), respectively.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Effect of HA-DTPH-PEGDA (PEGDA MW 3400Da) crosslinking density on collagen gel contraction. HA-DTPH-PEGDA constructs were prepared at crosslinking densities of 2:1, 6:1, and 12:1. (a) Images of the collagen/HA-DTPH-PEGDA composites at 48hours post-contraction, (b) quantification of gel areas – refer to Table 1 for contraction indices.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
Effect of PEGDA MWs on collagen gel contraction. HA-DTPH-PEGDA constructs were prepared using PEGDA MWs 258, 700, and 3400Da, while fixing the crosslinking density at 2:1.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
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6. Figure 5
Contraction of composite gels as a function of incubation time. HA-DTPH-PEGDA additives were prepared at crosslinking density of 2:1 using PEGDA MW 3400Da.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

7. Figure 6
Phalloidin-stained fibroblasts seeded within collagen and collagen/HA-DTPH-PEGDA gels. HA-DTPH was maintained at 0.25% (w/v), while PEGDA was added to obtain a 2:1 crosslinking density. No difference in cell morphology was observed with the addition of HA-DTPH-PEGDA. Average fibroblast density within control collagen gels increased drastically between 4 and 24hours post-contraction, whereas that within the composite gels remained largely unchanged. Bar=10μm.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
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8. Figure 7
Amount of HA extruded into the supernatant of different composite gels, as detected by the colorimetric HA-ELISA kit.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
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9. Figure 8
Thiol functionalized HA derivatives added to collagen gels. (a) Chemical structure of HA-DTPH and (b) chemical structure of PEGDA-crosslinked HA-DTPH hydrogel.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
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10. Figure 9 Schematic of the collagen gel contraction assay.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions