1. Differentiation potential of human muscle-derived cells towards chondrogenic phenotype in alginate beads culture 
R. Andriamanalijaona, Ph.D., E. Duval, M.Sc., M. Raoudi, Ph.D., S. Lecourt, Ph.D., J.T. Vilquin, Ph.D., J.P. Marolleau, M.D., J.P. Pujol, Ph.D., P. Galera, Ph.D., K. Boumediene, Ph.D. 
Osteoarthritis and Cartilage 
Volume 16, Issue 12, Pages (December 2008)
DOI: /j.joca
Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

2. Fig. 1
Alcian blue staining of MDCs cultured in alginate beads. MDCs (CD56− and CD56+ cells), cultured in alginate beads for chondrogenic differentiation, with or without TGFβ1 (ICM=ICM; T=ICM+TGFβ1), until 21 days, were stained with alcian blue for GAG detections. Immunohistochemical detection for ACP is shown in the same panel. Photographies' magnification, alcian blue: ×10; aggrecan: ×60. (A) CD56− cells; (B) CD56+ cells.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

3. Fig. 2
Expression of chondrogenesis markers in CD56− and CD56+ cells cultured in alginate beads under TGFβ1 treatment. Cells were cultured for differentiation as described in Material and methods during 3, 7, 14 and 21 days in chondrogenic medium (ICM, ) supplemented or not with 10ng/ml TGFβ1 (T, ■). Total RNA was extracted and real-time PCR was performed to measure mRNA levels for type IIA collagen (A1, C1), type IIB collagen (A2, C2) and ACP (B1, D1). All values were compared to undifferentiated cells (day 0), corresponding to monolayer culture in expanding medium. Protein extracts from undifferentiated cells (C0), from cells cultured in alginate beads and from HACs were analyzed by western-blot. Membranes were incubated with antibody directed towards human type II collagen (A3, C3) and human ACP (B2, D2). (A, B) CD56− cells; (C, D): CD56+ cells.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

4. Fig. 3
Expression of hypertrophic markers in CD56− cells. CD56− cells were cultured for differentiation as described in Material and methods during 3, 7 and 21 days in chondrogenic medium (ICM, ) supplemented or not with 10ng/ml TGFβ1 (T, ■). (A) Protein extracts from undifferentiated cells (C0), from cells cultured in alginate beads and from HACs were analyzed by western-blot. Membranes were incubated with antibody directed towards human type I (A1) and type X (A2) collagen. (B1) Cbfa1 mRNA was analyzed by real-time PCR. (B2) Nuclear extracts from undifferentiated cells or cells cultured in alginate beads during 3, 7, 14 and 21 days were used in EMSA using the Cbfa1 consensus sequence as a probe. Nuclear extracts from cells cultured 14 days in chondrogenic medium were used for DNA competition study using an excess of Cbfa1 consensus unlabeled probe (100-fold).
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

5. Fig. 4
Expression of hypertrophic markers in CD56+ cells. CD56+ cells were cultured for differentiation as described in Material and methods during 3, 7 and 21 days in chondrogenic medium (ICM, ) supplemented or not with 10ng/ml TGFβ1 (T, ■). (A) Protein extracts from undifferentiated cells (C0), from cells cultured in alginate beads and from HACs were analyzed by western-blot. Membranes were incubated with antibody directed towards human type I (A1) and type X (A2) collagen. (B1) Cbfa1 mRNA was analyzed by real-time PCR. (B2) Nuclear extracts from undifferentiated cells or cells cultured in alginate beads during 3, 7, 14 and 21 days were used in EMSA using the Cbfa1 consensus sequence as a probe. Nuclear extracts from cells cultured 14 days in chondrogenic medium were used for DNA competition study using an excess of Cbfa1 consensus unlabeled probe (100-fold).
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions