1. The Future of Phenotyping Research:
Biotic & Abiotic
Environment
Experiment-site
Plot
Plant
Organ
The Future of Phenotyping Research:
Connecting genotype to phenotype at all scales.
Rapid Phenotyping Working Group
Adapted from Gutierrez and Maere, (2014) Trends in Plant Science 19:5
TOOLS
MODELS 1

2. Prof. Margaret Frank, Cornell
Dani Martinez, Cornell
Margaret Krause, Cornell

3. Goal: automated vegetable crop grafter
Inefficient
Unreliable
Ungrafted Grafted

4. High-Throughput Automated Phenotyping System for Powdery Mildew Analysis
Affordable system for efficient phenotyping based on visual feature quantification from 1 cm2 biological samples.
Image acquisition:
Robotic stage to center samples and adjust focus.
UHR (46MP) images: Each pixel represents 1.44 µm2.
Focused images via image stacking from computed focal planes.
More than 1000 samples imaged per workday by using a single robot.
Image analysis:
Machine Learning procedures for detection of 6 μm wide fungal hyphae.
Quantifies Powdery mildew infection with a 91.7% correlation with human experts.
Disease progress:
2 days
4 days
6 days
9 days

5. Better yields FASTER and CHEAPER
Plant breeders have limited resources:
Land
Labor
Time
$$$
Seed
Solution: Predicting crop yields with UAVs
Fly UAV and capture images
Extract data from images
Predict yield and make selections
Margaret Krause, PhD Candidate, Plant Breeding