1. Association of a Nkx2-3 polymorphism with Crohn's disease and expression of Nkx2-3 is up-regulated in B cell lines and intestinal tissues with Crohn's disease 
Wei Yu, Zhenwu Lin, Ashley A. Kelly, John P. Hegarty, Lisa S. Poritz, Yunhua Wang, Tongyi Li, Stefan Schreiber, Walter A. Koltun 
Journal of Crohn's and Colitis 
Volume 3, Issue 3, Pages (September 2009)
DOI: /j.crohns
Copyright © 2009 European Crohn's and Colitis Organisation Terms and Conditions

2. Figure 1
Nkx2-3 mRNA expression is up-regulated in EBV-transformed B cells from CD patients. (A) Representative RT-PCR result: mRNA expression level of Nkx2-3 was increased in CD compared with non-CD sibling and healthy control. GAPDH expression served as a control. (B) Relative mRNA expression levels of Nkx2-3 of 16 sibling pairs and 2 healthy controls. The RT-PCR products bands on the photograph were scanned by densitometry. The relative mRNA expression level was expressed as Nkx2-3 compared to GAPDH. (C) Comparison of Nkx2-3 mRNA expression levels between CD patient and non-CD sibling in 16 sibling pairs. The values represent the fold change in densitometry data as described in (B). Between the Crohn's disease group and non-CD sibling group, the mRNA expression level of Nkx2-3 is significantly different (⁎⁎p<0.01). Data are presented as the means±standard errors.
Journal of Crohn's and Colitis 2009 3, DOI: ( /j.crohns )
Copyright © 2009 European Crohn's and Colitis Organisation Terms and Conditions

3. Figure 2
Nkx2-3 protein expression level is up-regulated in EBV-transformed B cells from CD patients. (A) Representative Western blot result: protein expression level of Nkx2-3 was increased in CD compared with non-CD sibling and healthy control. Actin expression served as a control. (B) Relative protein expression levels of Nkx2-3 of 16 sibling pairs and 2 healthy controls. The western blot bands on the photograph were scanned by densitometry. The relative protein expression level was expressed as Nkx2-3 compared to Actin. (C) Comparison of Nkx2-3 protein expression levels between CD patient and non-CD sibling in 16 sibling pairs. The values represent the fold change in densitometry data as described in (B). Between the Crohn's disease group and non-CD sibling group, the protein expression level of Nkx2-3 is significantly different (⁎⁎p<0.01). Data are presented as the means±standard errors.
Journal of Crohn's and Colitis 2009 3, DOI: ( /j.crohns )
Copyright © 2009 European Crohn's and Colitis Organisation Terms and Conditions

4. Figure 3
Nkx2-3 mRNA expression level is up-regulated in intestine tissues from Crohn's disease. (A) Representative RT-PCR result of one CD patient: mRNA expression level of Nkx2-3 was increased in diseased tissue compared with adjacent normal tissue. GAPDH expression served as a control. (B) mRNA expression levels of Nkx2-3 in 9 Crohn's disease patients. The RT-PCR products bands on the photograph were scanned by densitometry, the ratios of Nkx2-3 to GAPDH were calculated. For every patient, the ratio of Nkx2-3 to GAPDH with normal tissue was arbitrarily set as 1. The mRNA expression levels of Nkx2-3 were increased in diseased tissues compared with adjacent non-diseased tissues in 7 of 9 patients (77.8%).
Journal of Crohn's and Colitis 2009 3, DOI: ( /j.crohns )
Copyright © 2009 European Crohn's and Colitis Organisation Terms and Conditions