1. rDNA and CLONING

2. rDNA Stands for recombinant DNA
The scientist is “mixing” two different organisms DNA together
How is this able to work?
Because every organisms DNA is chemically the same
Adenine
Guanine
Cytosine
Thymine

3. rDNA Every living organism uses the same cellular processes of
Transcription
Translation
Protein Synthesis
GENE TO PROTEIN
Bacteria are very simple cells- easy to manipulate

4. Figure 13-9 Making Recombinant DNA
Section 13-3
Gene for human growth hormone
Recombinant DNA
Gene for human growth hormone
DNA recombination
Human Cell
Sticky ends
DNA insertion
Bacterial Cell
Bacterial chromosome
Bacterial cell for containing gene for human growth hormone
Plasmid

5. Flowchart Cloning Section 13-4
A body cell is taken from a donor animal.
An egg cell is taken from a donor animal.
The nucleus is removed from the egg.
The body cell and egg are fused by electric shock.
The fused cell begins dividing, becoming an embryo.
The embryo is implanted into the uterus of a foster mother.
The embryo develops into a cloned animal.

6. Figure 13-13 Cloning of the First Mammal
Section 13-4
A donor cell is taken from a sheep’s udder.
Donor Nucleus
These two cells are fused using an electric shock.
Fused Cell
Egg Cell
The nucleus of the egg cell is removed.
An egg cell is taken from an adult female sheep.
The fused cell begins dividing normally.
Cloned Lamb
Embryo
The embryo develops normally into a lamb—Dolly
The embryo is placed in the uterus of a foster mother.
Foster Mother