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Note the clone IDs used in the report duplicates are F176 and R551/F146 and F220 (see slides 10 and 11) CRN8 F118 F220 F283 R514 F146 F176 R582 R551.

Published byKaren Fleming Modified over 6 years ago

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Presentation on theme: "Note the clone IDs used in the report duplicates are F176 and R551/F146 and F220 (see slides 10 and 11) CRN8 F118 F220 F283 R514 F146 F176 R582 R551."— Presentation transcript:

1 Note the clone IDs used in the report duplicates are F176 and R551/F146 and F220 (see slides 10 and 11) CRN8 F118 F220 F283 R514 F146 F176 R582 R551

2 Report of Cahid Cakir on CRN8 deletions This was the basis for Figure 2 of van Damme et al. PLOS Pathogens 2012

3 Crn8 constructs (deletion and mutation)
Targeting Function SP LFLAK Effector GDV CRN8 1 17 49 439 599 F118 118 F118-M1 118 ??? F146 146 F176 176 F220 220 F283 283 R582 118 582 R551 118 551 R514 118 514

4 Crn8 constructs (Full Length)
Targeting Function SP LFLAK Effector CRN8 1 17 49 439 599 Crn8 PGR106

5 F118-Crn8 constructs without signal peptide and LFLAK
Targeting Function SP LFLAK Effector CRN8 1 17 49 439 599 F118 118 PGR106 F118 Crn2

6 F283-Crn8 constructs Targeting Function LFLAK CRN8 F283 PGR106 F118
SP LFLAK Effector DN CRN8 1 17 49 439 599 DN F283 283 PGR106 F118 F220 F283

7 F220-Crn8 constructs Targeting Function LFLAK CRN8 F220 PGR106 F118
SP LFLAK Effector DN CRN8 1 17 49 439 599 DN F220 220 PGR106 F118 F220 F283

8 R514-Crn8 constructs without signal peptide and LFLAK
Targeting Function SP LFLAK Effector CRN8 1 17 49 439 599 R514 118 439 R514 F118

9 New Deletion Analysis of Crn8

10 F118, F146 & F147 F118 118 F146 146 F176 176 F146 F118 F176

11 F118, F220 & R551 F118 118 F220 220 R551 118 551 F220 F118 R551

12 F118 & R582 F118 118 R582 118 582 R582 F118

13 Summary of results – from Cahid’s report

14 F118, F220 & R551 F118 118 F220 220 R551 118 551 F220 F118 R551

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