1. The Alarmin IL-33 Derived from HSV-2-Infected Keratinocytes Triggers Mast Cell- Mediated Antiviral Innate Immunity 
Rui Aoki, Tatsuyoshi Kawamura, Fumi Goshima, Youichi Ogawa, Susumu Nakae, Kohji Moriishi, Atsuhito Nakao, Shinji Shimada 
Journal of Investigative Dermatology 
Volume 136, Issue 6, Pages (June 2016)
DOI: /j.jid
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2. Figure 1
Functional IL-33 expression and production by HSV-2-infected keratinocytes. (a) Kinetic analysis of tissue levels of IL-33 and HSV-2 titer in the skin lysates of WT (B6) mice (n = 3) after HSV-2 infection at indicated time points. (b) Immunohistochemical staining for IL-33 in skin of WT mice at day 1 after intradermal infection with HSV-2. Original magnification ×200. Size bars = 100 μm. (c) BMMCs were stimulated with culture supernatants of lesional epidermal sheets obtained from WT (B6) mice (n = 3) or IL-33−/− mice (n = 3) intradermally injected with HSV-2, and analyzed for TNF-α production by ELISA. *P < 0.05 versus results for BMMCs treated with HSV-2-infected epidermis sup from IL-33−/− mice. **P < 0.01 versus results for BMMCs treated with uninfected epidermis sup from WT mice. BMMCs, bone marrow-derived mast cells; HSV-2, herpes simplex virus-2; TNF-α, tumor necrosis factor- α; WT, wild-type.
Journal of Investigative Dermatology  , DOI: ( /j.jid )
Copyright © 2016 The Authors Terms and Conditions

3. Figure 2
IL-33/ST2 signaling contributes to anti-HSV-2 host defense in vivo. Survival rates, clinical (EAE) scores, and lesion scores of WT (B6) mice and ST2−/− mice (a), or KitW/W-v mice, Kit+/+ mice, WT BMMC-reconstituted KitW/W-v mice, and ST2−/− BMMC-reconstituted KitW/W-v mice (b) at the indicated time points after intradermal injection with HSV-2 are shown. *P < 0.05 and **P < 0.01 versus WT mice (a) or KitW/W-v mice (b). Data are representative of at least two independent experiments; n = 7–10 mice/group. BMMCs, bone marrow-derived mast cells; EAE, experimental autoimmune encephalomyelitis; HSV-2, herpes simplex virus-2; WT, wild-type.
Journal of Investigative Dermatology  , DOI: ( /j.jid )
Copyright © 2016 The Authors Terms and Conditions