1. Volume 47, Issue 2, Pages 339-348.e4 (August 2017)
Nlrp6- and ASC-Dependent Inflammasomes Do Not Shape the Commensal Gut Microbiota Composition 
Michail Mamantopoulos, Francesca Ronchi, Filip Van Hauwermeiren, Sara Vieira-Silva, Bahtiyar Yilmaz, Liesbet Martens, Yvan Saeys, Stefan K. Drexler, Amir S. Yazdi, Jeroen Raes, Mohamed Lamkanfi, Kathy D. McCoy, Andy Wullaert 
Immunity 
Volume 47, Issue 2, Pages e4 (August 2017)
DOI: /j.immuni
Copyright © 2017 Elsevier Inc. Terms and Conditions

2. Figure 1
Gut Microbiota Variation in Unrelated C57BL/6J and Nlrp6−/− Mice Is Explained by Mother and Cage Co-variates but Not by Host Nlrp6 Genotype
(A and B) Relative abundances of indicated bacterial taxa in feces from C57BL/6J and Nlrp6−/− mice.
(C) Species diversity (Shannon index) of fecal samples from C57BL/6J and Nlrp6−/− mice.
(D) Unconstrained ordination (genus-level PCoA) of fecal microbiota composition from C57BL/6J and Nlrp6−/− mice, with color coding indicating the different cages (left) or the different mothers (right) of the respective mice.
(E and F) Genus-level distance-based redundancy analysis indicating the combined effect score (E) and the individual effect scores of the gut microbiota co-variates (F).
Statistics: (A, B) Mann-Whitney U with Benjamini-Hochberg correction for multiple variation; (C) Mann-Whitney U; (D–F) ordinations were performed on Bray-Curtis dissimilarity matrices calculated from normalized genus-level abundance profiles. C57BL/6J (n = 12), Nlrp6−/− (n = 12). ∗p < 0.05; ∗∗p < Data shown are representative for two independent cohorts. See also Figure S1 and Table S1.
Immunity  , e4DOI: ( /j.immuni )
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3. Figure 2
Separate Housing of Littermates Does Not Reveal a Host Genetic Effect of Nlrp6 on the Gut Microbiota Composition
(A–D) Relative abundances of indicated bacterial taxa (A and B); species diversity (Shannon index) (C); and unconstrained ordination (genus-level PCoA) with color coding indicating the different cages (left) or the different mothers (right) (D) of the fecal microbiota composition from Nlrp6+/+, Nlrp6+/−, and Nlrp6−/− littermates that were separately housed for 5 weeks.
(E–H) Relative abundances of indicated bacterial taxa (E and F); species diversity (Shannon index) (G); and unconstrained ordination (genus-level PCoA) with color coding indicating the different cages (left) or the different mothers (right) (H) of the fecal microbiota composition from Nlrp6+/+, Nlrp6+/−, and Nlrp6−/− littermates that were separately housed until 1 year of age.
(I) Genus-level distance-based redundancy analysis indicating the combined and the individual effect scores of the gut microbiota co-variates in the indicated littermate cohorts.
Statistics: (A, B, E, F) Mann-Whitney U with Benjamini-Hochberg correction for multiple variation; (C, G) Mann-Whitney U; (D, H, I) ordinations were performed on Bray-Curtis dissimilarity matrices calculated from normalized genus-level abundance profiles. 8- to 9-week-old littermates: Nlrp6+/+ (n = 21), Nlrp6+/− (n = 19), and Nlrp6−/− (n = 11); 1-year-old littermates: Nlrp6+/+ (n = 10), Nlrp6−/− (n = 9). Data shown are from one cohort. See also Figure S2 and Table S2.
Immunity  , e4DOI: ( /j.immuni )
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4. Figure 3
Lifetime Separation of Nlrp6+/+ and Nlrp6−/− Littermates Does Not Reveal a Host Genetic Effect of Nlrp6 on the Gut Microbiota Composition and Does Not Alter DSS Colitis Susceptibility
(A and B) Relative abundances of indicated bacterial taxa in feces from F2 Nlrp6+/+ and F2 Nlrp6−/− mice.
(C) Species diversity (Shannon index) of fecal samples from F2 Nlrp6+/+ and F2 Nlrp6−/− mice.
(D) Unconstrained ordination (genus-level PCoA) of fecal microbiota composition from F2 Nlrp6+/+ and F2 Nlrp6−/− mice, with color coding indicating the different cages (left) or the different mothers (right) of the respective mice.
(E) Genus-level distance-based redundancy analysis indicating the combined effect score and the individual effect scores of the gut microbiota co-variates.
(F–I) Body weight change (F), diarrhea score (G), rectal bleeding score (H), and colon length (I) of F2 Nlrp6+/+ and F2 Nlrp6−/− mice administered 2% DSS for 7 days followed by 2 days of normal drinking water.
Statistics: (A, B) Mann-Whitney U with Benjamini-Hochberg correction for multiple variation; (C) Mann-Whitney U; (D, E) ordinations were performed on Bray-Curtis dissimilarity matrices calculated from normalized genus-level abundance profiles. Data in (F)–(H) are presented as mean + SD. F2 Nlrp6+/+ (n = 10), F2 Nlrp6−/− (n = 12). Data shown in (A)–(E) are representative for two independent cohorts, data in (F)–(I) are from one cohort. See also Figure S3 and Table S3.
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5. Figure 4
ASC Host Genetic Status Does Not Influence Gut Microbiota Composition
(A–D) Relative abundances of indicated bacterial taxa (A and B); species diversity (Shannon index) (C); and unconstrained ordination (genus-level PCoA) (D) with color coding indicating the different cages (left) or the different mothers (right) of the fecal microbiota composition from Pycard+/+ and Pycard−/− littermates that were separately housed for 8 weeks.
(E–H) Relative abundances of indicated bacterial taxa (E and F); species diversity (Shannon index) (G); and unconstrained ordination (genus-level PCoA) (H) with color coding indicating the different cages (left) or the different mothers (right) of the fecal microbiota composition from Pycard+/− and Pycard−/− littermates that were separately housed for 15 weeks.
(I) Genus-level distance-based redundancy analysis indicating the combined and the individual effect scores of the gut microbiota co-variates in the indicated littermate cohorts.
Statistics: (A, B, E, F) Mann-Whitney U with Benjamini-Hochberg correction for multiple variation; (C, G) Mann-Whitney U; (D, H, I) ordinations were performed on Bray-Curtis dissimilarity matrices calculated from normalized genus-level abundance profiles. Pycard+/− (n = 18) and Pycard−/− (n = 13). Data shown are representative for two independent cohorts. See also Figure S4 and Table S4.
Immunity  , e4DOI: ( /j.immuni )
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