1. Altered cellular calcium regulatory systems in a rat model of cirrhotic cardiomyopathy 
Christopher A. Ward, Hongqun Liu, Samuel S. Lee 
Gastroenterology 
Volume 121, Issue 5, Pages (November 2001)
DOI: /gast
Copyright © 2001 American Gastroenterological Association Terms and Conditions

2. Fig. 1
Baseline and isoproterenol- and forskolin-stimulated isolated cardiomyocyte contractility in cirrhotic and control rats. Significantly different from corresponding baseline values: **P < 0.01.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

3. Fig. 2
A representative Western blot analysis of DHPR protein (α1 subunit) expression in BDL- and sham-treated hearts. Lanes 1 and 2, sham; lanes 3 and 4, BDL. Computerized optical densitometry showed significant differences between control (n = 6) and BDL-treated (n = 6) animals (P < 0.01).
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

4. Fig. 3
Comparison of L-type Ca2+ current densities from ventricular myocytes of BDL- and sham-treated animals. (A) Representative currents elicited by a depolarizing step to +10 mV from a conditioning potential of −40 mV. All currents were corrected for cell capacitance and expressed as current densities. (B) Current-voltage relationships were constructed by depolarizing steps to test potentials between −60 and +70 mV from the conditioning potential of −40 mV for myocytes isolated from BDL-treated (n = 19; ○) and sham-operated (n = 16; ●) animals.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

5. Fig. 4
Effect of isoproterenol on L-type Ca2+ currents in ventricular myocytes from BDL- and sham-treated animals. (A, B) Representative current traces of the effects of 10−8 mol/L isoproterenol on (A) sham- and (B) BDL-treated myocytes. (C, D) Representative data for the effects of 10−5 mol/L isoproterenol on (C) sham- and (D) BDL-treated myocytes. All currents were elicited by a depolarizing step to +10 mV from a conditioning potential of −40 mV and have been corrected in amplitude relative to individual cell ICa,L before addition of isoproterenol.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

6. Fig. 5
Effects of forskolin on L-type Ca2+ currents of ventricular myocytes from BDL- and sham-treated animals. Currents were elicited by a depolarizing step to +10 mV from a conditioning potential of −40 mV and have been corrected in amplitude relative to individual cell ICa,L before exposure to 10−5 mol/L forskolin.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

7. Fig. 6
RT-PCR photograph of RYR2 mRNA transcription from BDL- and sham-treated rat hearts using GAPDH as a positive control. Lane 1, -kb ladder; lanes 2 and 3, sham control; lanes 4 and 5, BDL. Computerized optical densitometry indicated no significant difference between sham-treated controls (n = 6) and BDL-treated animals (n = 6).
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

8. Fig. 7
RT-PCR photograph of sarcoplasmic reticulum Ca2+-pump ATPase (SERCA2) mRNA transcription from BDL- and sham-treated rat hearts using GAPDH as a positive control. Lane 1, 1-kb ladder; lanes 2 and 3, sham control; lanes 4 and 5, BDL. Computerized optical densitometry indicated no significant difference between sham-treated controls (n = 6) and BDL-treated animals (n = 6).
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

9. Fig. 8
Western blot analysis of RYR2, SERCA2, and Ca2+-binding protein (calsequestrin) expression in BDL- and sham-treated hearts. Lanes 1 and 2, sham; lanes 3 and 4, BDL. Computerized optical densitometry showed that there were no significant differences between sham-treated controls (n = 6) and BDL-treated animals (n = 6).
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

10. Fig. 9
Ryanodine receptor binding data in BDL and control groups. There were no significant differences in the mean values of the dissociation constant (Kd) and maximum density of binding sites (Bmax) between the 2 groups (n = 6 in each group).
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

11. Fig. 10
Ca2+ uptake in BDL and control groups. There were no significant differences in the mean values of the dissociation constant (Kd) and maximum density of binding sites (Bmax) between the 2 groups (n = 6 in each group).
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions