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Identification of susceptibility SNPs in IL10 and IL23R-IL12RB2 for Behçet's disease in Han Chinese  Hongsong Yu, PhD, Minming Zheng, MS, Lijun Zhang,

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Presentation on theme: "Identification of susceptibility SNPs in IL10 and IL23R-IL12RB2 for Behçet's disease in Han Chinese  Hongsong Yu, PhD, Minming Zheng, MS, Lijun Zhang,"— Presentation transcript:

1 Identification of susceptibility SNPs in IL10 and IL23R-IL12RB2 for Behçet's disease in Han Chinese 
Hongsong Yu, PhD, Minming Zheng, MS, Lijun Zhang, BS, Hua Li, PhD, Yunyun Zhu, BS, Ling Cheng, BS, Lin Li, MS, Bolin Deng, MS, Aize Kijlstra, PhD, Peizeng Yang, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 139, Issue 2, Pages (February 2017) DOI: /j.jaci Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 The influence of various rs genotypes on the mRNA expression of IL23R. A, Expression of IL23R in nonstimulated PBMCs from normal controls carrying various genotypes of rs (n = 11 for the CC genotype, n = 11 for the CG genotype, and n = 11 for the GG genotype). B, Expression of IL23R in anti-CD3/CD28 antibodies-stimulated PBMCs from healthy controls carrying various genotypes of rs (n = 11 for the CC genotype, n = 11 for the CG genotype, and n = 11 for the GG genotype). Real-time PCR was conducted to detect gene expression, and the relative expression levels of IL23R gene were quantified by using the 2−ΔΔCt method. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Comparison of the relative expression levels of IL10 variants. A, Expression of IL10 in nonstimulated PBMCs from healthy controls with various rs genotypes (GG = 11, GT = 11, TT = 11). B, Expression of IL10 in anti-CD3/CD28 antibodies-stimulated PBMCs from healthy controls carrying various genotypes of rs (GG = 11, GT = 11, TT = 11). Real-time PCR was performed to detect gene expression, and the relative expression levels of IL10 gene were quantified by using the 2−ΔΔCt method. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 The influence of various rs genotypes on IL-17 production (n = 11 for the CC genotype, n = 11 for the CG genotype, and n = 11 for the GG genotype). The production of IL-17 was quantified by ELISA in PBMCs, which were obtained from healthy controls with various rs genotypes and treated with anti-CD3/CD28 antibodies. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 The influence of various rs genotypes on IL-10 production (n = 11 for the GG genotype, n = 11 for the GT genotype, and n = 11 for the TT genotype). The production of IL-10 was measured by ELISA in PBMCs, which were obtained from healthy controls carrying various genotypes of rs and treated with anti-CD3/CD28 antibodies. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig E1A Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig E1B Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig E2A Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

9 Fig E2B Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

10 Fig E3A Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

11 Fig E3B Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

12 Fig E4A Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

13 Fig E4B Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

14 Fig E5 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

15 Fig E6 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

16 Fig E7 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

17 Fig E8 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

18 Fig E9 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

19 Fig E10 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions


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