Presentation is loading. Please wait.

Presentation is loading. Please wait.

Differential Modulation of Ku70/80 DNA-Binding Activity in a Patient with Multiple Basal Cell Carcinomas  Paola Mazzarelli, Carla Rabitti, Paola Parrella,

Published byAugustus Hodge Modified over 5 years ago

Similar presentations

Presentation on theme: "Differential Modulation of Ku70/80 DNA-Binding Activity in a Patient with Multiple Basal Cell Carcinomas  Paola Mazzarelli, Carla Rabitti, Paola Parrella,"— Presentation transcript:

1 Differential Modulation of Ku70/80 DNA-Binding Activity in a Patient with Multiple Basal Cell Carcinomas  Paola Mazzarelli, Carla Rabitti, Paola Parrella, Davide Seripa, Paolo Persichetti, Giovanni Francesco Marangi, Giuseppe Perrone, Maria Luana Poeta, Mario Delfino, Vito Michele Fazio, MD  Journal of Investigative Dermatology  Volume 121, Issue 3, Pages (September 2003) DOI: /j x Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Expression of p53, Ki67-MIB1, and bcl-2 in two BCC. Tissue sections from superficial (T3) and infiltrative (T4) tumors from the patient are shown as examples for the differential expression of p53, Ki67, and bcl-2 proteins. Positive nuclear staining for p53 is evident in both nonaggressive and aggressive BCC but it is absent from the surrounding normal skin. In the superficial tumor (T3) many nuclei do not express Ki67. The pattern of expression for bcl-2 is exclusively cytoplasmic in the epithelial cells. There is no detectable staining for bcl-2 in the infiltrative (T4) tumor. Arrows: positively stained lymphocytes are shown as control. Magnification: 400 ×. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 (a) Ku70/80 DNA-binding activity and protein expression in normal and tumor samples. Representative results of the electrophoretic mobility shift assay of protein-DNA complexes are shown. Arrows indicate free probe and specific protein-DNA complexes. From left to right: Sk1, Sk2, normal skin samples from the patient; T1-T5, nuclear extracts from five different BCC from the patient; CaCo, CaCo2 cell line protein extract (HTB 37, ATCC); C1-C5, normal skin samples from healthy individuals; p, free probe without protein extract; C6-C9, bladder mucosa and mammary gland from healthy individuals. (b) Supershift experiments. From left to right: lane 1, protein extract from normal skin without antibodies; lane 2, polyclonal anti-Ku70 antibody incubated with protein extract from normal skin; lane 3, anti-Ku86 antibodies incubated with nuclear extracts from normal skin sample; p, free probe. (c) Western blot analysis of p70 and p80 expression. Sk1, normal skin from the patient; T1-T4, BCC from the patient. The upper panel shows results from experiment 1; the exposure time allows the detection of the Ku80 protein only for the T4 tumor. (arrow) The lower panel shows results from experiment 2; a longer exposure time allows the detection of both Ku70 and Ku80 proteins. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Nuclear expression of Ku70, Ku86, and DNA-PKcs in no aggressive (T2) and aggressive (T4, T5) tumors. Representative results of paraffin sections stained with antibodies against Ku70, Ku86, and DNA-PKcs. Ku86 immunostaining is weaker than for Ku70 for all the tumors tested. There are no significant differences in protein expression between the two aggressive BCC (T4, T5) compared to the nonaggressive BCC. Magnification: 400 ×. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Download ppt "Differential Modulation of Ku70/80 DNA-Binding Activity in a Patient with Multiple Basal Cell Carcinomas  Paola Mazzarelli, Carla Rabitti, Paola Parrella,"

Similar presentations

Volume 114, Issue 5, Pages (May 1998)

Volume 114, Issue 5, Pages (May 1998)
Expression of the Hypoxia-Inducible and Tumor-Associated Carbonic Anhydrases in Ductal Carcinoma in Situ of the Breast  Charles C. Wykoff, Nigel Beasley,

Expression of the Hypoxia-Inducible and Tumor-Associated Carbonic Anhydrases in Ductal Carcinoma in Situ of the Breast  Charles C. Wykoff, Nigel Beasley,
NF-κB Accumulation Associated with COL1A1 Transactivators Defects during Chronological Aging Represses Type I Collagen Expression through a –112/–61-bp.

NF-κB Accumulation Associated with COL1A1 Transactivators Defects during Chronological Aging Represses Type I Collagen Expression through a –112/–61-bp.
Expression of Purinergic Receptors in Non-melanoma Skin Cancers and Their Functional Roles in A431 Cells  Aina V.H. Greig, Geoffrey Burnstock  Journal.

Expression of Purinergic Receptors in Non-melanoma Skin Cancers and Their Functional Roles in A431 Cells  Aina V.H. Greig, Geoffrey Burnstock  Journal.
TSLP Down-Regulates S100A7 and ß-Defensin 2 Via the JAK2/STAT3-Dependent Mechanism  Hana Lee, Woo-In Ryu, Hee Joo Kim, Hyun Cheol Bae, Hwa Jung Ryu, Jung.

TSLP Down-Regulates S100A7 and ß-Defensin 2 Via the JAK2/STAT3-Dependent Mechanism  Hana Lee, Woo-In Ryu, Hee Joo Kim, Hyun Cheol Bae, Hwa Jung Ryu, Jung.
Expression of Transglutaminase 5 in Normal and Pathologic Human Epidermis  Eleonora Candi, Sergio Oddi, Andrea Paradisi, Alessandro Terrinoni, Marco Ranalli,

Expression of Transglutaminase 5 in Normal and Pathologic Human Epidermis  Eleonora Candi, Sergio Oddi, Andrea Paradisi, Alessandro Terrinoni, Marco Ranalli,
Requirement of heat shock protein 90 in mesangial cell mitogenesis

Requirement of heat shock protein 90 in mesangial cell mitogenesis
Human Skin is a Steroidogenic Tissue: Steroidogenic Enzymes and Cofactors Are Expressed in Epidermis, Normal Sebocytes, and an Immortalized Sebocyte Cell.

Human Skin is a Steroidogenic Tissue: Steroidogenic Enzymes and Cofactors Are Expressed in Epidermis, Normal Sebocytes, and an Immortalized Sebocyte Cell.
Basement Membrane Zone Remodeling During Appendageal Development in Human Fetal Skin. The Absence of Type VII Collagen is Associated with Gelatinase-A.

Basement Membrane Zone Remodeling During Appendageal Development in Human Fetal Skin. The Absence of Type VII Collagen is Associated with Gelatinase-A.
Heme-Scavenging Role of α1-Microglobulin in Chronic Ulcers

Heme-Scavenging Role of α1-Microglobulin in Chronic Ulcers
Ahmad Waseem, Yasmin Alam, Anand Lalli 

Ahmad Waseem, Yasmin Alam, Anand Lalli 
Rose-Anne Romano, Barbara Birkaya, Satrajit Sinha 

Rose-Anne Romano, Barbara Birkaya, Satrajit Sinha 
Vamsidhar Velcheti, MD, David L. Rimm, MD, PhD, Kurt A

Vamsidhar Velcheti, MD, David L. Rimm, MD, PhD, Kurt A
Volume 115, Issue 1, Pages (July 1998)

Volume 115, Issue 1, Pages (July 1998)
Colocalization of Kindlin-1, Kindlin-2, and Migfilin at Keratinocyte Focal Adhesion and Relevance to the Pathophysiology of Kindler Syndrome  J.E. Lai-Cheong,

Colocalization of Kindlin-1, Kindlin-2, and Migfilin at Keratinocyte Focal Adhesion and Relevance to the Pathophysiology of Kindler Syndrome  J.E. Lai-Cheong,
Herpes Simplex Virus Associated Erythema Multiforme (HAEM) is Mechanistically Distinct from Drug-Induced Erythema Multiforme: Interferon-γ is Expressed.

Herpes Simplex Virus Associated Erythema Multiforme (HAEM) is Mechanistically Distinct from Drug-Induced Erythema Multiforme: Interferon-γ is Expressed.
Activation of Epidermal Growth Factor Receptor/ERK Signaling Correlates with Suppressed Differentiation in Malignant Acanthosis Nigricans  Ingo Haase,

Activation of Epidermal Growth Factor Receptor/ERK Signaling Correlates with Suppressed Differentiation in Malignant Acanthosis Nigricans  Ingo Haase,
Ultraviolet A Radiation-Induced Immediate Iron Release Is a Key Modulator of the Activation of NF-κB in Human Skin Fibroblasts  Olivier Reelfs, Rex M.

Ultraviolet A Radiation-Induced Immediate Iron Release Is a Key Modulator of the Activation of NF-κB in Human Skin Fibroblasts  Olivier Reelfs, Rex M.
Challenges and Pitfalls in HNPCC Screening by Microsatellite Analysis and Immunohistochemistry  Annegret Müller, Giuseppe Giuffre, Tina Bocker Edmonston,

Challenges and Pitfalls in HNPCC Screening by Microsatellite Analysis and Immunohistochemistry  Annegret Müller, Giuseppe Giuffre, Tina Bocker Edmonston,
High Frequency of Loss of Heterozygosity on Chromosome Region 9p21–p22 but Lack of p16INK4a/p19ARF Mutations in Greek Patients with Basal Cell Carcinoma.

High Frequency of Loss of Heterozygosity on Chromosome Region 9p21–p22 but Lack of p16INK4a/p19ARF Mutations in Greek Patients with Basal Cell Carcinoma.

Similar presentations

Ads by Google