Welcome to the PROTEIN SHOW! T A G C With your host, RNA POLYMERASE!

Welcome to the Cytoplasm!
U C G Hello, my name is RIBOSOME I am a PROTEIN FACTORY Now, why did we come to the cytoplasm? To find the RIBOSOME, so it will build the protein coded for by this piece of mRNA.

PLAYERS… This is the mRNA. What does it do?
U C G This is the mRNA. What does it do? A U Amino Acid This is the tRNA. What does it do? PLAYERS… This is the ribosome. What does it do? A Site P Site The tRNA brings specific amino acids to the ribosome. The amino acids are used to build the protein. The mRNA brings in the instructions from the DNA to BUILD the protein. The ribosome is a mixture of RNA and protein, and READS the mRNA, and builds the protein.

mRNA Anatomy… CODON… any 3 mRNA bases. Each CODON codes for 1 AMINO ACID. A U C G

Ribosome Anatomy… The P SITE, where tRNA molecules first arrive
The A SITE, where tRNA molecules are moved A Site P Site The bigger protein subunit The smaller protein subunit

tRNA Anatomy… The amino acid that is carried by the tRNA.
A long, coiled piece of RNA A U A single, MAGNIFIED part of the RNA, called the ANTI-CODON

TRANSLATION A U Amino Acid 1 A Site P Site A U C G

TRANSLATION A U C G Amino Acid 1 A Site P Site

TRANSLATION C U G Amino Acid 2 A U Amino Acid 1 A Site P Site A U C G

TRANSLATION Amino Acid 1 C U G Amino Acid 2 A Site P Site A U A U C G

TRANSLATION PEPTIDE BOND Amino Acid 1 Amino Acid 2 A Site P Site C U G

TRANSLATION Amino Acid 1 C U G Amino Acid 2 A Site P Site A U A U C G

TRANSLATION Amino Acid 1 C U G Acid 2 A Site P Site A U A U C G

TRANSLATION Amino Acid 1 C U G Acid 2 A U A Site P Site A U C G

TRANSLATION Amino Acid Amino Acid 1 Acid 2 A Site P Site G A C U G A U

TRANSLATION Amino Acid 3 Amino Acid 1 Acid 2 A Site P Site G A C U G A

TRANSLATION Amino Acid 1 Acid 2 Amino Acid 3 A Site P Site C U G G A A

TRANSLATION Amino Acid 1 Acid 2 Amino Acid 3 A Site P Site G A C U G A

Amino Acid 1 Acid 2 Acid 3 TRANSLATION A Site P Site G A C U G A U C G

TRANSLATION Amino Acid 1 Amino Acid 2 Amino Acid 3 A Site P Site G A C
U G A U C G

TRANSLATION Amino Acid 1 Amino Acid 2 Amino Acid 3 A Site P Site C U G

TRANSLATION Amino Acid Amino Acid 1 Amino Acid 2 Amino Acid 3 A Site
U A Amino Acid Amino Acid 1 TRANSLATION Amino Acid 2 Amino Acid 3 C U G A Site P Site G A A U C G

TRANSLATION Amino Acid 4 Amino Acid 1 Amino Acid 2 Amino Acid 3 A Site
U A Amino Acid 4 Amino Acid 1 TRANSLATION Amino Acid 2 C U G Amino Acid 3 A Site P Site G A A U C G

U A Amino Acid 4 Amino Acid 1 TRANSLATION Amino Acid 2 Amino Acid 3 A Site P Site G A A U C G

U A Amino Acid 4 Amino Acid 2 Amino Acid 3 A Site P Site G A A U C G

U A Amino Acid 4 Amino Acid 3 A Site P Site G A A U C G

U A Amino Acid 4 A Site P Site G A A U C G

TRANSLATION Amino Acid 1 Acid 2 Acid 3 Amino Acid 4 A Site P Site C U
G A A U C G

TRANSLATION Amino Acid 1 Acid 2 Acid 3 Amino Acid 4 A Site P Site C U
G A A Site P Site A U C G

TRANSLATION Amino Acid 5 Amino Acid 1 Acid 2 Acid 3 Amino Acid 4
G A C Amino Acid 5 Amino Acid 1 Acid 2 Acid 3 TRANSLATION C U A Amino Acid 4 G A A Site P Site A U C G

TRANSLATION Amino Acid 5 Amino Acid 1 Acid 2 Acid 3 Amino Acid 4
G A C Amino Acid 5 Amino Acid 1 Acid 2 Acid 3 TRANSLATION G A C U A Amino Acid 4 A Site P Site A U C G

TRANSLATION Amino Acid 1 Acid 2 Acid 3 Amino Acid 5 Amino Acid 4
G A C Amino Acid 5 TRANSLATION C U A Amino Acid 4 A Site P Site A U C G

TRANSLATION Amino Acid 1 Acid 2 Acid 3 Amino Acid 5 Amino Acid 4
G A C Amino Acid 5 C U A Amino Acid 4 A Site P Site A U C G

TRANSLATION Amino Acid 1 Acid 2 Acid 3 Acid 4 Amino Acid 5 A Site
G A C Amino Acid 5 A Site P Site C U A A U C G

TRANSLATION Amino Acid 1 Acid 2 Acid 3 Acid 4 Amino Acid 5 A Site
G A C Amino Acid 5 C U A A Site P Site A U C G

TRANSLATION Amino Acid 6 Amino Acid 1 Acid 2 Acid 3 Acid 4 Amino
U Amino Acid 6 Amino Acid 1 Acid 2 Acid 3 Acid 4 TRANSLATION G A C Amino Acid 5 C U A A Site P Site A U C G

TRANSLATION Amino Acid 1 Acid 2 Acid 3 Acid 4 Amino Acid 6 Amino
U Amino Acid 6 TRANSLATION G A C Amino Acid 5 C U A A Site P Site A U C G

U Amino Acid 6 TRANSLATION C U A G A C Amino Acid 5 A Site P Site A U C G

U Amino Acid 6 TRANSLATION G A C Amino Acid 5 A Site P Site A U C G

U Amino Acid 6 G A C Amino Acid 5 A Site P Site A U C G

TRANSLATION Amino Acid 1 Acid 2 Acid 3 Acid 4 Acid 5 Amino Acid 6
U Amino Acid 6 A Site P Site G A C A U C G

U Amino Acid 6 G A C A Site P Site A U C G

TRANSLATION Amino Acid 1 Acid 2 Acid 3 Acid 4 Acid 5 STOP Amino Acid 6
U A STOP TRANSLATION A U Amino Acid 6 G A C A Site P Site A U C G

U A STOP A U Amino Acid 6 G A C A Site P Site A U C G

U A STOP A U Amino Acid 6 A Site P Site A U C G

TRANSLATION Amino Acid 1 Acid 2 Acid 3 Acid 4 Acid 5 Amino Acid 6 STOP
U Amino Acid 6 C U A STOP A Site P Site A U C G

U Amino Acid 6 A Site P Site C U A STOP A U C G

TRANSLATION Amino Acid 1 Acid 2 Acid 3 Acid 4 Acid 5 Acid 6 A Site
P Site C U A STOP A U A U C G

P Site A U C U A STOP A U C G

U A Site P Site C U A STOP A U C G

TRANSLATION A U C U A STOP A Site P Site A U C G

TRANSLATION C U A STOP A Site P Site A U C G

TRANSLATION is finished!
Amino Acid 1 Acid 2 Acid 3 Acid 4 Acid 5 Acid 6 TRANSLATION is finished! BYE BYE! What happened to the finished protein? It goes off into your body to do its job, whatever that is. A Site P Site

Welcome to the PROTEIN SHOW! T A G C With your host, RNA POLYMERASE!

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Welcome to the PROTEIN SHOW! T A G C With your host, RNA POLYMERASE!

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