1. Splenic CD169+ macrophages trap and prevent early Lm replication and spread.
Splenic CD169+ macrophages trap and prevent early Lm replication and spread. (A) Confocal image (left) and isosurfaced image (right) of intracellular Lm located within a CD169+ macrophage in the MZ. Scale bars, 10 μm. 3D, three-dimensional. (B) Quantification of intracellular Lm within CD169+ macrophages in the spleens of WT mice. Each symbol represents a distinct region of the spleen. Data are shown as means ± SEM. (C) Quantification of viable Lm from sorted WT CD169+ macrophages at 1 hpi. Data are shown as means ± SEM. Representative example of gating strategy used to sort CD169+ macrophages from the spleen. CFU, colony-forming units; MZMs, MZ macrophages; UV, ultraviolet; FSC-A, forward scatter–area; FSC-H, forward scatter–height; SSC-A, side scatter–area; PE, phycoerythrin. (D) Confocal images of spleen sections from WT or CD169-DTR mice. Lm are rendered with spots. Scale bars, 50 μm. (E) Survival of WT or CD169-DTR mice (***P < , Mantel-Cox log-rank test). (F) Quantification of Lm numbers from the spleens of WT or CD169-DTR mice at 10 hpi. Each symbol represents an individual mouse, shown as means ± SEM (*P < 0.05, Mann-Whitney test). (G) Quantification of Lm in the blood of WT or CD169-DTR mice. Each symbol represents an individual mouse, shown as mean ± SEM (*P < 0.05, Mann-Whitney test). All data are representative of two to three separate experiments with three to six mice per group.
Oriana A. Perez et al. Sci. Immunol. 2017;2:eaah5520
Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works