1. Mitochondrial Gene Polymorphisms That Protect Mice From Colitis
Florian Bär, Wiebke Bochmann, Andrea Widok, Kilian von Medem, Rene Pagel, Misa Hirose, Xinhua Yu, Kathrin Kalies, Peter König, Ruwen Böhm, Thomas Herdegen, Anna T. Reinicke, Jürgen Büning, Hendrik Lehnert, Klaus Fellermann, Saleh Ibrahim, Christian Sina 
Gastroenterology 
Volume 145, Issue 5, Pages e3 (November 2013)
DOI: /j.gastro
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2. Figure 1
Baseline characteristics of mucosal OXPHOS capacity in CS mice. (A) Comparison of ADP and ATP content in intestinal crypt isolates of B6.B6, B6.AKR, B6.NOD, and B6.NZB mice presented as relative light units (RLU)/250,000 crypts. (B) Comparison of reactive oxygen species (ROS) content in intestinal crypt isolates of B6.B6, B6.AKR, B6.NOD, and B6.NZB mice presented as RLU/250,000 crypts. (C) Enzymatic complex activity of OXPHOS complexes I, II, and IV expressed in relative units. (D) Representative picture of exemplary proteins of the 5 OXPHOS complexes determined by Western blot analysis. (E) Analysis of the mt-CO1, NDUFB8, and mt-ND3 protein levels as representatives of complexes I and IV after determination of the blots' optical densities by using ImageJ software. (F) Complex IV activity in isolated intestinal crypts was determined 20 minutes after stimulation with DNP. All experiments were performed in at least 6 different animals per strain. Values are shown as mean ± SEM (*P < .05; **P < .01).
Gastroenterology  , e3DOI: ( /j.gastro )
Copyright © 2013 AGA Institute Terms and Conditions

3. Figure 2
Mitochondrial DNA variants influence susceptibility against chronic DSS colitis. (A) Modified disease activity index alongside 30 days of chronic DSS colitis calculated from body weight loss and occult blood test. (B) Colon length after 30 days of chronic DSS colitis. (C) Evaluation of disease activity by mouse endoscopy, the murine endoscopic index of colitis severity (MEICS) was calculated as described in the Materials and Methods section. (D) Representative endoluminal pictures (upper panel) and H&E-stained tissue specimens (lower panel) obtained from mice at day 30 of the experiment are shown. (E) The calculation of the histologic score was performed according to an established scoring system as described in the Materials and Methods section. DSS colitis experiments were repeated twice. In each experiment, 5 male and 5 female mice per strain were examined. Values are shown as mean ± SEM (*P < .05; **P < .01).
Gastroenterology  , e3DOI: ( /j.gastro )
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4. Figure 3
Mitochondrial DNA variants influence susceptibility against TNBS colitis. (A) Development of body weight during TNBS-induced colitis. (B) Evaluation of disease activity by mouse endoscopy, the murine endoscopic index of colitis severity (MEICS) was calculated as described in the Materials and Methods section. (C) Representative endoluminal pictures (upper panel) and H&E-stained tissue specimens (lower panel) obtained from mice at day 10 of the experiment. (D) The calculation of the histologic score was performed according to an established scoring system as described in the Materials and Methods section. TNBS colitis experiments were performed twice. In each experiment, 5 male and 5 female mice per strain were examined. Values are shown as mean ± SEM (*P < .05; **P < .01).
Gastroenterology  , e3DOI: ( /j.gastro )
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5. Figure 4
Altered physiology of primary intestinal epithelial cells in CS mice. (A) Representative pictures of Ki-67–stained colon samples of BL6.BL6, BL6.AKR, BL6.NOD, and BL6.NZB mice. (B) Determination of mucosal cell proliferation by counting Ki-67–positive stained cells per high-power field (HPF) (magnification, 400×). (C) Representative pictures of cleaved caspase-3 immunofluorescence analysis in colonic cross-sections of CS mice at baseline level. (D) Quantification of cleaved caspase-3–positive stained cells per crypt in 5 different colonic cross-sections per animal. (E) Western blot analysis of phosphorylated IκB-α, IκB-α, cleaved caspase-3, and β-actin. Representative blots are shown. (F) Analysis of the ratio between phosphorylated IκB-α and nonphosphorylated IκB-α after determination of the blots optical densities by using ImageJ software. All experiments were performed in at least 6 different animals per strain. Values are shown as mean ± SEM (*P < .05; **P < .01).
Gastroenterology  , e3DOI: ( /j.gastro )
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6. Supplementary Figure 1
Morphologic and physiologic baseline conditions in CS mice. (A) Weight development of different CS mice from week 5 to week 15. (B) Representative pictures of colonoscopy (upper panels) and histology (lower panels) of the 4 different mice strains under baseline conditions. (C) Enzymatic complex activity of OXPHOS complex V expressed in relative units. (D) Representative pictures of colon slides immunostained with anti-Nd3. (E) Comparison of the ratio of DNA quantities of mitochondrial-specific coenzyme 1 in relation to the nuclear 18s RNA gene determined by polymerase chain reaction (PCR). (F) Crypt length of the different CS mice. All experiments were performed in at least 6 different animals per strain. Values are shown as mean ± SEM (*P < .05; **P < .01).
Gastroenterology  , e3DOI: ( /j.gastro )
Copyright © 2013 AGA Institute Terms and Conditions

7. Supplementary Figure 2
(A) Fold-change of the expression profile of NF-κB downstream genes (Icam-1 and Kc) in the respective mouse strains (n = 6 per each strain). Values are shown as mean ± SEM (*P < .05; **P < .01).
Gastroenterology  , e3DOI: ( /j.gastro )
Copyright © 2013 AGA Institute Terms and Conditions