1. Nucleotide excision repair polymorphisms, polycyclic aromatic hydrocarbon exposure, and their effects on sperm deoxyribonucleic acid damage and male factor infertility 
Aihua Gu, M.D., Ph.D., Guixiang Ji, Ph.D., Pengfei Zhu, M.S., Yong Zhou, Ph.D., Guangbo Fu, M.D., Yankai Xia, Ph.D., Ling Song, M.S., Shoulin Wang, Ph.D., Xinru Wang, Ph.D. 
Fertility and Sterility 
Volume 94, Issue 7, Pages e5 (December 2010)
DOI: /j.fertnstert
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

2. Sperm PAH-DNA adducts were detected by an immunofluorescence staining with phycoerythrin-labeled antibody (orange); sperm DNA was counterstained with 6-diamino-2-phenylindole (DAPI) (blue). (A, C, E) The sperm staining of an infertile subject. (B, D, E) The same sample treated with 10 μmol/L benzo-(α)-pyrene (BPDE), set up as the positive control.
Fertility and Sterility  , e5DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

3. Detection of sperm DNA damage with use of TUNEL assay
Detection of sperm DNA damage with use of TUNEL assay. Histogram data of M2 values represented the fluorescein isothiocyanate conjugate fluorescence-labeled DNA-damaged spermatozoa. Two samples with TUNEL values of (A) 5.4% and (B) 24.9% are illustrated.
Fertility and Sterility  , e5DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

4. Damage in DNA in ejaculates from 620 patients
Damage in DNA in ejaculates from 620 patients. (A) TUNEL value without logarithmic transformation. (B) TUNEL value with logarithmic transformation (ln TUNEL).
Fertility and Sterility  , e5DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

5. Distribution of urine CR-adjusted 1-hydroxypyrene (1-OHP) levels in 620 patients. (A) 1-hydroxypyrene levels without logarithmic transformation. (B) 1-Hydroxypyrene levels with logarithmic transformation.
Fertility and Sterility  , e5DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

6. Association between logarithm of the sperm DNA damage and logarithm of urinary 1-hydroxypyrene (1-OHP). Adjusting for age, smoking, drinking, tea consumption, vitamin use, and abstinence time, the urinary 1-hydroxypyrene levels were associated significantly with sperm DNA damage with use of a linear regression: β coefficients = 0.183, P<.001.
Fertility and Sterility  , e5DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions