Presentation is loading. Please wait.

Presentation is loading. Please wait.

Toluene downregulates filaggrin expression via the extracellular signal-regulated kinase and signal transducer and activator of transcription–dependent.

Published byShinta Setiawan Modified over 5 years ago

Similar presentations

Presentation on theme: "Toluene downregulates filaggrin expression via the extracellular signal-regulated kinase and signal transducer and activator of transcription–dependent."— Presentation transcript:

1 Toluene downregulates filaggrin expression via the extracellular signal-regulated kinase and signal transducer and activator of transcription–dependent pathways  Hana Lee, PhD, Jung Jin Shin, MD, Hyun Cheol Bae, PhD, Woo-In Ryu, PhD, Sang Wook Son, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 139, Issue 1, Pages e5 (January 2017) DOI: /j.jaci Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Toluene inhibits filaggrin expression in keratinocytes in a dose-dependent manner. Filaggrin expression was decreased by toluene exposure at the mRNA (A) and protein level (B) in keratinocytes. Downregulated filaggrin protein expression was detected by immunofluorescence staining in HSEMs (C). HSEM, Human skin equivalent model. Columns and error bars represent means ± SEMs from 3 independent experiments. *P < .05. Bar = 50 μm. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Toluene induces the downregulation of filaggrin expression by ERK/STAT-3– dependent mechanism. Toluene increased the ERK phosphorylation in keratinocytes (A). Inhibition of ERK restored the toluene-mediated downregulation of filaggrin expression shown by Western blot (B) and immunofluorescence assay (C). Phosphorylated STAT-3 protein expression was increased after toluene exposure (D). Downregulated filaggrin expression was upregulated by STAT3 siRNA at the promoter activity (E), protein level (F), and after toluene exposure. Results of chromatin immunoprecipitation assay showed that the STAT-3 binds directly to the putative STAT-3 binding sequence of the filaggrin promoter (G). DAPI, 4′-6-Diamidino-2-phenylindole, dihydrochloride; DMSO, dimethyl sulfoxide. Columns and error bars represent means ± SEMs from 3 independent experiments. *P < .05. Bar = 100 μm. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig E1 NMF: relationship with toluene exposure. Toluene-treated HSEMs were lysed in 0.1 mol KOH and subjected to HPLC. Exposure to toluene resulted in a 40% and 60% reduction in the PCA and UCA content, respectively. The PCA levels were 42.3 ± 3.43 and 26.6 ± 4.41 μg/mL for control and toluene, respectively (A). The respective UCA levels were 4.1 ± 0.05 and 1.4 ± 0.03 μg/mL for control and toluene, respectively (B). *P < .001. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig E2 Suppression of p38 with SB or JNK with SP does not change filaggrin expression. Toluene did not increase p38 phosphorylation and JNK phosphorylation in keratinocytes. Keratinocytes were preincubated with the specific inhibitors against ERK (U0126), p38 (SB239063), and JNK (SP600125) before stimulation with toluene. Western blot analysis characterized the expression of total ERK, pERK (Thr202/Tyr204), total p38 MAPK, pp38 MAPK (Thr180/Tyr182), total JNK, pJNK (Thr183/Tyr185), and filaggrin (A). Also, the expression of filaggrin was visualized by IF staining (B). Data are representative of 3 independent experiments. Bar = 100 μm. DAPI, 4′-6-Diamidino-2-phenylindole, dihydrochloride; IF, immunofluorescence; MAPK, mitogen-activated protein kinase. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig E3 ERK and STAT3 siRNA block the toluene-mediated downregulation of filaggrin expression. Keratinocytes were transfected with ERK siRNA (A) and STAT3 siRNA (B) and then exposed to toluene. STAT3 and ERK siRNA block the toluene-mediated downregulation of filaggrin expression. Schematic diagram shows the position and directions of PCR primers used for detecting the products of the chromatin immunoprecipitation (ChIP) assay (C). Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Toluene downregulates filaggrin expression via the extracellular signal-regulated kinase and signal transducer and activator of transcription–dependent."

Similar presentations

Proinflammatory cytokine–induced and chemical mediator–induced IL-8 expression in human bronchial epithelial cells through p38 mitogen-activated protein.

Proinflammatory cytokine–induced and chemical mediator–induced IL-8 expression in human bronchial epithelial cells through p38 mitogen-activated protein.
TNF-like weak inducer of apoptosis (TWEAK) and TNF-α cooperate in the induction of keratinocyte apoptosis  Maya Zimmermann, PhD, Andrea Koreck, MD, Norbert.

TNF-like weak inducer of apoptosis (TWEAK) and TNF-α cooperate in the induction of keratinocyte apoptosis  Maya Zimmermann, PhD, Andrea Koreck, MD, Norbert.
IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway  Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,

IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway  Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,
Phosphatidylinositol 3-kinase and p38 mitogen-activated protein kinase regulate induction of Mcl-1 and survival in glucocorticoid-treated human neutrophils 

Phosphatidylinositol 3-kinase and p38 mitogen-activated protein kinase regulate induction of Mcl-1 and survival in glucocorticoid-treated human neutrophils 
Rhinovirus infection causes steroid resistance in airway epithelium through nuclear factor κB and c-Jun N-terminal kinase activation  Alberto Papi, MD,

Rhinovirus infection causes steroid resistance in airway epithelium through nuclear factor κB and c-Jun N-terminal kinase activation  Alberto Papi, MD,
Pharmacological inhibition of caspase-8 suppresses inflammation-induced lymphangiogenesis and allograft rejection in the cornea  Wenru Su, MD, PhD, Longhui.

Pharmacological inhibition of caspase-8 suppresses inflammation-induced lymphangiogenesis and allograft rejection in the cornea  Wenru Su, MD, PhD, Longhui.
Low E-prostanoid 2 receptor levels and deficient induction of the IL-1β/IL-1 type I receptor/COX-2 pathway: Vicious circle in patients with aspirin-exacerbated.

Low E-prostanoid 2 receptor levels and deficient induction of the IL-1β/IL-1 type I receptor/COX-2 pathway: Vicious circle in patients with aspirin-exacerbated.
Laurent L'homme, PhD, David Dombrowicz, PhD 

Laurent L'homme, PhD, David Dombrowicz, PhD 
Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.

Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.
Thymic stromal lymphopoietin downregulates filaggrin expression by signal transducer and activator of transcription 3 (STAT3) and extracellular signal-regulated.

Thymic stromal lymphopoietin downregulates filaggrin expression by signal transducer and activator of transcription 3 (STAT3) and extracellular signal-regulated.
Airway microbiome and responses to corticosteroids in corticosteroid-resistant asthma patients treated with acid suppression medications  Elena Goleva,

Airway microbiome and responses to corticosteroids in corticosteroid-resistant asthma patients treated with acid suppression medications  Elena Goleva,
Requirement of the NF-κB pathway for induction of Wnt-5A by interleukin-1β in condylar chondrocytes of the temporomandibular joint: functional crosstalk.

Requirement of the NF-κB pathway for induction of Wnt-5A by interleukin-1β in condylar chondrocytes of the temporomandibular joint: functional crosstalk.
Yeonsue Jang, Sang H. Jeong, Yoon-Hee Park, Hyun C

Yeonsue Jang, Sang H. Jeong, Yoon-Hee Park, Hyun C
Phosphatidylinositol 3-kinase and p38 mitogen-activated protein kinase regulate induction of Mcl-1 and survival in glucocorticoid-treated human neutrophils 

Phosphatidylinositol 3-kinase and p38 mitogen-activated protein kinase regulate induction of Mcl-1 and survival in glucocorticoid-treated human neutrophils 
TSLP Down-Regulates S100A7 and ß-Defensin 2 Via the JAK2/STAT3-Dependent Mechanism  Hana Lee, Woo-In Ryu, Hee Joo Kim, Hyun Cheol Bae, Hwa Jung Ryu, Jung.

TSLP Down-Regulates S100A7 and ß-Defensin 2 Via the JAK2/STAT3-Dependent Mechanism  Hana Lee, Woo-In Ryu, Hee Joo Kim, Hyun Cheol Bae, Hwa Jung Ryu, Jung.
Julie M. Caldwell, PhD, Carine Blanchard, PhD, Margaret H

Julie M. Caldwell, PhD, Carine Blanchard, PhD, Margaret H
IFN-γ orchestrates mesenchymal stem cell plasticity through the signal transducer and activator of transcription 1 and 3 and mammalian target of rapamycin.

IFN-γ orchestrates mesenchymal stem cell plasticity through the signal transducer and activator of transcription 1 and 3 and mammalian target of rapamycin.
Reduction of CRKL expression in patients with partial DiGeorge syndrome is associated with impairment of T-cell functions  Mauro Giacomelli, PhD, Rajesh.

Reduction of CRKL expression in patients with partial DiGeorge syndrome is associated with impairment of T-cell functions  Mauro Giacomelli, PhD, Rajesh.
IL-4 confers resistance to IL-27–mediated suppression on CD4+ T cells by impairing signal transducer and activator of transcription 1 signaling  Zhihong.

IL-4 confers resistance to IL-27–mediated suppression on CD4+ T cells by impairing signal transducer and activator of transcription 1 signaling  Zhihong.
Arachidonic acid regulation of the cytosolic phospholipase A2α/cyclooxygenase-2 pathway in mouse endometrial stromal cells  Zhen-Ao Zhao, Ph.D., Zhi-Rong.

Arachidonic acid regulation of the cytosolic phospholipase A2α/cyclooxygenase-2 pathway in mouse endometrial stromal cells  Zhen-Ao Zhao, Ph.D., Zhi-Rong.

Similar presentations

Ads by Google