1. Serological Pipets Blue Pump 2mL, Green Pump 10mL , Red Pump 25 mL
Never ever mouth pipet
Solutions should never touch the cotton plug
Pipetting should always be done at eye level
Pipet always be in a vertical position
Numbers represent how much you have pipetted
Example if I fill the pipet to the 0 line and pipet 1mL the meniscus will touch the 1 mL line
*** Pipets on Practical will only have the reverse numbers

2. Serological Pipets
Without Touching the pipet tip, remove it from the plastics sleeve
Insert the wide end into the pipet pump
Twist the pipet gently into the pump to get a tight seal
Fill the pipet a bit above the amount desired and then slowly lower the meniscus to that line.
Remove the pipet and discard it into an appropriate discard container.  
Pipet can be reused for same solution only!!!!

3. Serological Pipet Basics

4. Lab 3A Measuring small volumes
Solutions add across the data tables
You must uses the different color dyes to get the final solution
You will compare your final solution to mine and it should be the same volume and roughly the same color.
Red
Blue
Green
Yellow
Purple

5. Using a Micropipette

6. Choosing and Setting a Micropipette
Choose the smallest pipet that can measure your volume
Set the pipet
Numbers get larger when you move the dial to the right
Numbers get smaller when you move the dial to the left

7. Setting Micropipet Practice
For each of the following-Draw the correct setting!!!
P20 set to 15.85
P100 set to 56.5
P1000 set to 265
P200 set to 19.5
P20 set to 3.5

9. Using a Centrifuge
TAB
Put the tubes opposite each other in the centrifuge.
Tubes should be loaded with tab facing the inside
Make a counterbalance for the centrifuge tube you want to put in the centrifuge.
The masses, not volumes, of the tubes should be as close as possible!
Unbalanced tubes may permanently damage the centrifuge
If balanced it will be almost silent

10. Using a Centrifuge Close the lid, will turn on if balanced
Do not open the lid until it stops spinning completely
Remove the tubes carefully after the centrifuge so they don’t get mixed up again
Most often used to separate suspensions in liquids.
Also used to mix small amounts of liquid and relocate to bottom of tube.

11. Using a Centrifuge

12. During the Practical You Should
One set of solutions per Lab Station (17 tubes)
ALL solutions must be done by the end of class 11/7.
Prelab for 3E (M/V) and 3F (%/M/V)
Do not do the actual Biuret testing until the lab on 11/16
Prelab for 3H (C1V1=C2V2)
Make all solutions for 3E, 3F and 3H
Calculations only for 3G (Molarity)
Don’t forget TLABS (review Sheets)

13. Using a GLX Using a Colorimeter
Remember Copper Sulfate and Heavy medals must go in the waste container in the hood

14. Background on spectrophotometer

15. A spectrophotometer is a device used to measure light at a specific wavelength. It consists of two parts: a spectrometer and a photometer. The spectrometer provides light at a specific wavelength. The photometer measures how intense the light is. By calculating the amount of light that a solution is able to absorb and applying Beer's Law, the spectrophotometer can determine the concentration of a colored solution.

16. Spectrophotometer video
12zs