1. Figure S2 Supplementary data a b CCA1 CCA2 CCA4 CCA3 CCA5 EGFR HER2
Figure S2. EGFR and c-Myc proteins are overexpressed to similar levels in F0 and corresponding F1 tumors. (a) IHC staining demonstrates relatively high levels of EGFR expression in CCA1, 3 and 4. And moderate levels of c-Myc expression in F0 and F1 in CCA1 and CCA2 tumors. Scale bar = 20µm. Total expression index values are shown below each image. (b) Normal pancreas tissue expresses moderate levels of EGFR, but no detectable levels of HER2 or c-Myc protein. Scale bar for 20x=20µm; for 40x=10µm. Expression indices of EGFR, HER2 and c-Myc calculated as described in Materials and Methods. a b
CCA1
CCA2
CCA4
CCA3
CCA5
EGFR
HER2
c-Myc
Primary
Tumor (F0)
PDX (F1)
< <
< <50
< <
< <50
20x x
150