Presentation is loading. Please wait.

Presentation is loading. Please wait.

Rapid Diagnosis of α-Thalassemia by Melting Curve Analysis

Published byAntonia Mary Park Modified over 5 years ago

Similar presentations

Presentation on theme: "Rapid Diagnosis of α-Thalassemia by Melting Curve Analysis"— Presentation transcript:

1 Rapid Diagnosis of α-Thalassemia by Melting Curve Analysis
Thongperm Munkongdee, Phantip Vattanaviboon, Parichut Thummarati, Paijit Sewamart, Pranee Winichagoon, Suthat Fucharoen, Saovaros Svasti  The Journal of Molecular Diagnostics  Volume 12, Issue 3, Pages (May 2010) DOI: /jmoldx Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Schematic representation of the α-globin gene cluster and relative positions of the seven primers used in the melting curve analysis. The black boxes indicate the extent of the α-thalassemia 1, −−SEA, and −−THAI deletions and α-thalassemia 2, −α3.7, and −α4.2 deletions. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 The melting curves of the amplified α-thalassemia and normal PCR products representing −−SEA deletions, −−THAI deletions, α2-globin fragment, and Ψζ-globin fragment. These amplicons were differentiated according to their characteristic Tm. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

4 Figure 3 Examples of the melting curve analysis of individuals with various α-globin genotypes. A: Two peaks of α2-globin and Ψζ-globin fragments from normal globin genotype. B: Three peaks of α2-globin, −−SEA, and Ψζ-globin from SEA α-thalassemia 1 trait. C: Three peaks of −−THAI, α2-globin, and Ψζ-globin from Thai α-thalassemia 1 trait. D: A single peak of −−SEA from Hb Bart's hydrops fetalis. E: A single peak of −α3.7 from homozygous α-thalassemia 2. F: Two peaks of −−SEA and Ψζ-globin from deletional Hb H disease. G: Two peaks of −−THAI and Ψζ-globin from deletional Hb H disease. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

5 Figure 4 Replicated melting curve analysis of SEA α-thalassemia 1 trait. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Download ppt "Rapid Diagnosis of α-Thalassemia by Melting Curve Analysis"

Similar presentations

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.
A Multiplex qPCR Gene Dosage Assay for Rapid Genotyping and Large-Scale Population Screening for Deletional α-Thalassemia Wanjun Zhou, Ge Wang, Xuefeng.

A Multiplex qPCR Gene Dosage Assay for Rapid Genotyping and Large-Scale Population Screening for Deletional α-Thalassemia Wanjun Zhou, Ge Wang, Xuefeng.
Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,

Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,
CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.

CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.
Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.

Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.
Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis  Philippe Szankasi, David.

Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis  Philippe Szankasi, David.
Simple, Efficient, and Cost-Effective Multiplex Genotyping with Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of Hemoglobin.

Simple, Efficient, and Cost-Effective Multiplex Genotyping with Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of Hemoglobin.
EGFR Mutations in Lung Adenocarcinomas

EGFR Mutations in Lung Adenocarcinomas
Detection of Exon 12 Mutations in the JAK2 Gene

Detection of Exon 12 Mutations in the JAK2 Gene
Statistical Considerations for Immunohistochemistry Panel Development after Gene Expression Profiling of Human Cancers  Rebecca A. Betensky, Catherine.

Statistical Considerations for Immunohistochemistry Panel Development after Gene Expression Profiling of Human Cancers  Rebecca A. Betensky, Catherine.
Simultaneous Genotyping of α-Thalassemia Deletional and Nondeletional Mutations by Real-Time PCR–Based Multicolor Melting Curve Analysis  Qiuying Huang,

Simultaneous Genotyping of α-Thalassemia Deletional and Nondeletional Mutations by Real-Time PCR–Based Multicolor Melting Curve Analysis  Qiuying Huang,
Toward Universal Flavivirus Identification by Mass Cataloging

Toward Universal Flavivirus Identification by Mass Cataloging
LightCycler Technology in Molecular Diagnostics

LightCycler Technology in Molecular Diagnostics
Molecular Genetics of Pediatric Soft Tissue Tumors

Molecular Genetics of Pediatric Soft Tissue Tumors
Yanggu Shi, Sharon F. Terry, Patrick F. Terry, Lionel G

Yanggu Shi, Sharon F. Terry, Patrick F. Terry, Lionel G
Simultaneous Genotyping of GSTT1 and GSTM1 Null Polymorphisms by Melting Curve Analysis in Presence of SYBR Green I  Fátima Marín, Nadia García, Xavier.

Simultaneous Genotyping of GSTT1 and GSTM1 Null Polymorphisms by Melting Curve Analysis in Presence of SYBR Green I  Fátima Marín, Nadia García, Xavier.
Michael T. Seipp, Jacob D. Durtschi, Michael A

Michael T. Seipp, Jacob D. Durtschi, Michael A
Comparison of High-Resolution Melting Analysis, TaqMan Allelic Discrimination Assay, and Sanger Sequencing for Clopidogrel Efficacy Genotyping in Routine.

Comparison of High-Resolution Melting Analysis, TaqMan Allelic Discrimination Assay, and Sanger Sequencing for Clopidogrel Efficacy Genotyping in Routine.
Homogeneous Polymerase Chain Reaction Nucleobase Quenching Assay to Detect the 1-kbp Deletion in CLN3 That Causes Batten Disease  Paul G. Rothberg, Denia.

Homogeneous Polymerase Chain Reaction Nucleobase Quenching Assay to Detect the 1-kbp Deletion in CLN3 That Causes Batten Disease  Paul G. Rothberg, Denia.
Nucleotide Extension Genotyping by High-Resolution Melting

Nucleotide Extension Genotyping by High-Resolution Melting

Similar presentations

Ads by Google