1. How to Make and Use a DNA Fragment Standard Curve

2. How to Make and Use a DNA Fragment Standard Curve

3. Purpose of the DNA Standard Curve
To determine the fragment sizes (base pairs) in an unknown sample
The marker/ladder (control) is used to prepare a standard curve
Provides a standard for comparison to the unknown fragment sizes
Known as called "interpolation

4. How are Standard Curves Created?
Begin with a set of standards of a known quantity
Relate this known quantity to something we can measure
Graph the relationship to create a standard curve
Determine size of an unknown DNA fragment

5. bottom
____
edge of the well.
the bottom

6. A picture of the gel is taken and used to make the measurements
bottom edge

7. bottom edge

9. Start with the smallest fragment and continue to plot the larger fragment sizes until all are plotted. Draw a best fit line through the center
of the points.

10. Do not draw a line through each point.

11. “interpolation”