1. Smaller T cell zone FRC areas in aged spleens.
Smaller T cell zone FRC areas in aged spleens. (A) Image analysis pipeline for splenic FRC quantification. Representative images from young (top) and aged (bottom) splenic white pulp. FRCs were identified by expression of PDPN (left). A digital rendering of the PDPN staining was created using Imaris isosurface function (middle). Combined image of CD8α (yellow), B220 (blue), CD31 (red), and PDPN (green) (right). Scale bar, 50 μm. (B) PDPN area quantification was accomplished by averaging the area of four different white pulp 20× z stack images (1-μm step size, four steps) per mouse (six mice per group) (C) Longitudinal images of entire spleens from naive young (top) and aged (bottom) mice. Scale bar, 200 μm. Data are representative of n = 6 mice per group. Error bars = ±SEM. Statistical significance was determined by two-tailed t test. **p < 0.01.
April R. Masters et al. ImmunoHorizons 2018;2:
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