1. Exposure to rSema4A increases Treg cell numbers in human PBMC in vitro cultures.
Exposure to rSema4A increases Treg cell numbers in human PBMC in vitro cultures. Human PBMCs were cultured with or without external rSema4A for 48 h. Mouse IgG1 served as irrelevant protein control. After harvest, the numbers of CD4+ T cells and Treg cells were assessed by flow cytometry using specific Abs to corresponding cell surface and intracellular molecules. CD3+CD4high cells shown on upper panel dot plots (A) were further selected to evaluate the relative number of Treg cells in each in vitro experimental setting (B). The Foxp3high cells were gated on CD4+Foxp3+ dot plots. (C) CD25+ expression on CD4+Foxp3high Treg cells is depicted on the corresponding cell density plots, whereas NRP-1 expression (dot plots) is shown above. Data are representative from three independent experiments. *p < 0.04, **p <
Svetlana P. Chapoval et al. ImmunoHorizons 2019;3:71-87
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