1. Impaired development of Treg cell function upon overexpression of FOXP3A384T in naïve CD4+CD25− T cells.
Impaired development of Treg cell function upon overexpression of FOXP3A384T in naïve CD4+CD25− T cells. (A) Schematic representation of the constructs used in transduction. (B) Expression levels of FOXP3, CD25, and CD127 in transduced cells on day 28 post-transduction. (C) Cytokine production by transduced cells expressing WT or mutated FOXP3. (D and E) Suppressive potency of empty vector (EV)–, FOXP3WT-, FOXP3A384T-, and FOXP3R397W-transduced cells. Transduced, GFP+ cells were cocultured with proliferation dye–labeled CD4+CD25− Teff cells at a 1:4 ratio. Results are representative of 11 independent assessments on seven independent transduction experiments carried out on cells from four different donors. All data sets were compared with one-way nonparametric ANOVA followed by Dunn’s posttest.
Khalid Bin Dhuban et al. Sci. Immunol. 2017;2:eaai9297
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