1. Fig. 2. Rapid immunochromatography for specific detection of DENV NS1 proteins (serotypes 1 to 4) and ZIKV NS1 protein.
Rapid immunochromatography for specific detection of DENV NS1 proteins (serotypes 1 to 4) and ZIKV NS1 protein. (A and B) Images of rapid test strips. Strip numbers refer to the DENV serotype NS1 (1 to 4), pan-DENV (P; all four DENV serotype NS1 proteins), or ZIKV NS1 (Z) detected. rNS1 proteins, indicated with an “r” preceding the virus name, were prepared at 500 ng/ml, and the strips were run using 50 μl of solution. Strip #1 (detects DENV1), mAb pair 912/271; strip #2 (detects DENV2), mAb pair 243/1; strip #3 (detects DENV3), mAb pair 411/55; strip #4 (detects DENV4), mAb pair 626/55; strip P (“pan-DENV”; detects all four DENV serotypes), mAb pair /323; strip Z (detects ZIKV), mAb pair 130/110. The test proteins run on the strips are recombinant DENV NS1 serotypes 1 to 4 (rDENV1 to rDENV4), as well as rNS1 proteins from ZIKV (rZIKV), rWNV, rYFV, rJEV, and rTBEV. C, control; NS1, detection site for specific NS1 protein. (C to E) Limits of detection for viral NS1 proteins using the serotype-specific (SSp) DENV strips 1 to 4 (C), the pan-DENV strip (D), and the ZIKV strip (E). The limits of detection, representing three independent determinations, are recorded in the figures. Each point (C to E) is presented as the means and SD. (F) NS1-containing supernatants from Vero cells infected with DENV1–4 (Vs DENV1–4) or ZIKV [Vs ZIKV-U (Uganda) or ZIKV-B (Brazil)] were chromatographed on strips 1 to 4, pan-DENV (P), or ZIKV (Z) NS1 strips. The arrows indicate the strips with positive NS1 signals. Horizontal test lines (F) result from applying antibodies to the nitrocellulose using a mechanical striper device; the circular dot signals result from applying antibodies to the nitrocellulose using a standard pipettor.
Irene Bosch et al., Sci Transl Med 2017;9:eaan1589
Published by AAAS