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Identification of IgE-binding components of citrus red mite in sera of patients with citrus red mite–induced asthma  Hee-Yeon Kim, MDa, Hae-Sim Park,

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Presentation on theme: "Identification of IgE-binding components of citrus red mite in sera of patients with citrus red mite–induced asthma  Hee-Yeon Kim, MDa, Hae-Sim Park,"— Presentation transcript:

1 Identification of IgE-binding components of citrus red mite in sera of patients with citrus red mite–induced asthma  Hee-Yeon Kim, MDa, Hae-Sim Park, MDa, Yoon-Keun Kim, MDb, Jee-Woong Son, MDb, Hyun-Ah Kim, MSa, Jung-Hee Suh, MSa, Dong-Ho Nahm, MDa, Sang-Heon Cho, MDb, Kyung-Up Min, MDb, You-Young Kim, MDb  Journal of Allergy and Clinical Immunology  Volume 107, Issue 2, Pages (February 2001) DOI: /mai Copyright © 2001 Mosby, Inc. Terms and Conditions

2 Fig. 1 Protein staining of 12% SDS-PAGE of CRM extract. MW, Molecular weight. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2001 Mosby, Inc. Terms and Conditions

3 Fig. 2 A, Binding pattern of IgE-binding components of CRM extract in 10 individual patients (lanes 1-10) , control sera (lane C) , and buffer (lane B) . M, Marker protein. B, Percentage of sera of CRM-allergic individuals showing specific IgE binding to the different proteins of CRM. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2001 Mosby, Inc. Terms and Conditions

4 Fig. 3 Two-dimensional PAGE analysis of CRM extract. A, Coomassie blue staining of 2-dimensional PAGE; B, IgE immunoblotting of 2-dimensional PAGE with the reactive pooled sera. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2001 Mosby, Inc. Terms and Conditions

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