1. Purified natural and recombinant Fel d 1 and cat albumin in in vitro diagnostics for cat allergy 
Ronald van Ree, PhDa, W.Astrid van Leeuwen, a, Ingrid Bulder, BSca, Julian Bond, PhDb, Rob C. Aalberse, PhDa 
Journal of Allergy and Clinical Immunology 
Volume 104, Issue 6, Pages (December 1999)
DOI: /S (99)
Copyright © 1999 Mosby, Inc. Terms and Conditions

2. Fig. 1
RAST analysis with purified natural and recombinant allergens. A, For 509 sera, RAST results are compared for cat extract and the combination of nFel d 1 and nCA. Results are expressed in RAST units per milliliter. B, For 493 sera, RAST results are compared for natural and recombinant Fel d 1. Results are expressed in RAST units per milliliter. C, For 77 sera, RAST results are compared for nCA (25 μg/test) and rCA (0.5 μg/test).
Journal of Allergy and Clinical Immunology  , DOI: ( /S (99) )
Copyright © 1999 Mosby, Inc. Terms and Conditions

3. Fig. 2
SDS-PAGE/silver staining of nCA and rCA. Natural and recombinant albumin were both analyzed by SDS-PAGE/silver staining. nCA demonstrated a major band of greater than 60 kd. Both high and low molecular weight (MW) contaminations were detected. Culture supernatant–containing rCA is shown in lane 3 . A major band at the same molecular weight as nCA was observed. A contaminating band just below rCA was also present in control culture supernatant (not shown). Molecular weight markers contain a 10-kd ladder.
Journal of Allergy and Clinical Immunology  , DOI: ( /S (99) )
Copyright © 1999 Mosby, Inc. Terms and Conditions

4. Fig. 3
Comparison of IgE reactivity with nCA and rCA by means of immunoblot. Thirteen sera were analyzed on immunoblot for IgE reactivity with nCA and rCA. Sera can roughly be divided in 3 different reactivity patterns with both versions of albumin (A , B , and C ). Only the first group (left 5 lanes) recognized nCA and rCA (A) . None of the discrepant sera recognized CA at all (B and C ). For reference purposes, mAb 4E9 reactivity is shown in the last lane.
Journal of Allergy and Clinical Immunology  , DOI: ( /S (99) )
Copyright © 1999 Mosby, Inc. Terms and Conditions

5. Fig. 4
RAST inhibition with nCA and rCA. A, A serum pool was used in a cat RAST inhibition to compare the inhibitory potency of cat extract, nFel d 1, and nCA. Results are expressed as percentage of inhibition. B, Same analysis as A , but with rFel d 1 and rCA instead of natural allergens. C, nFel d 1 RAST inhibition with the serum pool and nFel d 1 and rFel d 1 as inhibitors. D, Sera No. 364 (concordant) and No. 459 (discrepant) were used in a RAST inhibition with nCA on the solid phase (0.5 μg/test) and nCA or rCA as the inhibitor. Results are expressed as percentage of inhibition.
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Journal of Allergy and Clinical Immunology  , DOI: ( /S (99) )
Copyright © 1999 Mosby, Inc. Terms and Conditions