1. AbH AbF p=0.01 p=0.02 5 p=0.004 p=0.001 Fold increase in 4
NHS
NHS+IgG
NHS+AbH
no serum
HI NHS
Fold increase in
C3 deposition 1 2 3 4 5
p=0.02
p=0.001
AbH
p=0.01
p=0.004
NHS+AbF
AbF
Supplementary Figure 2. Deposition of C3-related fragments on A549 lung cancer cells: Effect of autoantibodies F and H. Antibodies tested are IgG and human anti-CFH autoantibody from patients F and H (0.2 mg/ml), in the presence of normal human serum (NHS) as a source of complement or heat inactivated serum (HI-NHS). Fold increase in C3 deposition is reported relative to the baseline observed in the absence of serum.