1. Proteomic Study Reveals Plasma Protein Changes in Congenital Heart Diseases 
Chao Xuan, MD, PhD, Ge Gao, MD, PhD, Qin Yang, MD, PhD, Xiu-Li Wang, MD, Zhi-Gang Liu, MD, PhD, Xiao-Cheng Liu, MD, Guo- Wei He, MD, PhD 
The Annals of Thoracic Surgery 
Volume 97, Issue 4, Pages (April 2014)
DOI: /j.athoracsur
Copyright © 2014 The Society of Thoracic Surgeons Terms and Conditions

2. Fig 1
Representative two-dimensional gel electrophoresis is shown of plasma from patients with congenital heart disease and normal controls. (A) Plasma protein (500 μg) from patients with (left) tetralogy of Fallot (TOF), (center) normal controls, and (right) ventricular septal defect (VSD) was subjected to two-dimensional electrophoresis with first-dimension pH gradient of 3 to 10 and second-dimension sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Gels were stained with colloidal Coomassie Blue R-250. Spots were excised, processed as described in the experimental procedures, and analyzed by peptide mass fingerprinting. Square boxes indicate the location of gelsolin or Ficolin-3. (B) The intensity of plasma gelsolin was shown by the significant differences in expression between (left) TOF patients (8,962.7 ± 445.7) and (right) normal controls (12,119.0 ± 402.0; p<0.01 by unpaired t test). (C) The intensity of plasma ficolin-3 in TOF (7,477.2 ± 1,058.6 vs in control; p<0.01 by unpaired t test) and VSD (4,629.0 ± vs in control; p<0.01 by unpaired t test) groups was shown downregulated in expression compared with the normal controls (15,239.8 ± 147.4). Data are shown as mean ± standard error of the mean.
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )
Copyright © 2014 The Society of Thoracic Surgeons Terms and Conditions

3. Fig 2
Enzyme-linked immunosorbent assay was used to measure plasma levels of gelsolin and ficolin-3. (A) Plasma gelsolin levels in tetralogy of Fallot (TOF) patients (76.30 ± 4.42 μg/mL) were significantly depressed compared with the healthy controls (131.80 ± 23.46 μg/mL; p = by unpaired t test). (B) The plasma ficolin-3 level in normal controls ( ± μg/mL) was significantly elevated compared with the patients with TOF (4.930 ± 0.355 μg/mL; p = by unpaired t test) and patients with isolated ventricular septal defect (VSD; ± μg/mL vs control; p = by unpaired t test). Data are shown as mean ± standard error of the mean (n = 40).
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )
Copyright © 2014 The Society of Thoracic Surgeons Terms and Conditions