1. How to measure tear film Quality & Quantity the least INVASIVE way.
Vision4You
How to measure tear film Quality & Quantity the least INVASIVE way.
Claes Feinbaum M.Sc. Ph.D.
Professor Emeritus OPHTHALMOLOGY & OPTOMETRY
BEN GURION UNIVERSITY OF THE NEGEV, ISRAEL

2. Introduction
The last three decades have seen the awareness of dry eye dis-ease (DED) rise considerably around the world. Through the mutual efforts of many organizations, much has been learned about the basis and impact of this disease in the continued attempt to improve clinical care for affected individuals.

3. TFOS DEWS II revised definition
Dry eye is a multifactorial disease of the ocular surface charac-terized by a loss of homeostasis of the tear film, and accompanied by ocular symptoms, in which tear film instability and hyper-osmolarity, ocular surface inflammation and damage, and neuro-sensory abnormalities play etiological roles.

4. Ocular surface
Is defined as comprising the structures of the eye and adnexa, including the cornea, con-junctiva, eyelids, eyelashes, tear film, main and accessory lacrimal glands, and the meibomian glands. Thus, the tears, both in terms of the individual components at the site of production, and as a film on the ocular surface, are included within the term “ocular surface

5. The Tear Film

6. Glycocalix
The innermost layer of the tear film connects the glycocalyx of corneal epithelia with mucins secreted by goblet cells. This mucous layer is critical in adhering the tear film to the cornea. It connects the hydrophobic cells of the cornea with the hydrophilic aqueous layer of the tear film.
The innermost layer of the tear film connects the glycocalyx of corneal epithelia with mucins secreted by goblet cells. This mucous layer is critical in adhering the tear film to the cornea. It connects the hydrophobic cells of the cornea with the hydrophilic aqueous layer of the tear film.

7. Typically tear film abnormalities associated
With the corneal glycocalyx layer are localized to discrete areas of the cornea, whereas those associated with mucins are more diffuse. Common problems that disrupt the glycocalyx include corneal sequestrums, ulcerations, touch and mineralizations. For example, a corneal ulceration generates a dry spot that will retain debris and bacteria that would normally be cleared by an intact tear film.

8. Goblet cells
Produce most of the mucins within the tear film, but some are derived from corneal epithelia. The act of blinking spreads these mucins across the corneal surface. Mucins are "sticky" hydrophilic carbohydrates that form a physical barrier to bacterial adherence. The initial stages of clearing bacteria from the ocular surface include mucins coating the invaders. Mucins are therefore critical in preventing bacterial infection of the eye.

9. The Blepharitis Patient
The chronic blepharitis patient that has attempted numerous unsuccessful treatment regimens and has visited numerous eye doctors looking for some relief. Interestingly, the cause of their chronic blepharitis might be secondary to Demodex infestation.

10. Demodex
The Demodex mite is an eight-legged (an arachnid) ectoparasite (living on the surface of the host) that can reside in our hair follicles and sebaceous glands. Of the 65 described Demodex species, only Demodex brevis and Demodex folliculorum are found on humans. Demodex is contracted and spread by either direct contact or dust containing eggs.

11. DES or KCS
Dry eye syndrome (DES) is one of the most frequently encountered ocular morbidities. Twenty-five percent of patients who visit ophthalmic clinics report symptoms of dry eye, making it a growing public health problem and one of the most common conditions seen by eye care practitioners. The prevalence of DES is estimated to be 7.4% to 33.7% depending on which study is cited, how the disease is diagnosed, and which population is surveyed.

12. What is the impact of dry eye syndrome?
More than three million American women over the age of 50 have moderate to severe dry eyes, while more than 1.5 million American men over the age of 50 are similarly afflicted. This condition is fairly benign and easily treated with artificial tears, however. Many contact lens wearers experience dry eyes at some point with many discontinuing or limiting lens wear. Dry eye is a significant finding in patients having undergone refractive surgery, especially LASIK.

13. Topofilm

14. TEAR FILM STABILITY TESTS

15. Normal tear film is continuously available
Normal tear film is continuously available. Blinking maintains the tear film continuity. However if you keep your eyes open long enough, without blinking, the tear film will start breaking up. Your eye will feel uncomfortable forcing you to blink. In patients with dry eyes the tear film is unstable, and breaks up faster. Therefore the tear break up time in patients who have dry eyes is shorter.

16. Tear Film break-up time (TFBUT or FBUT)
This measures the interval between the individual’s last complete blink and the break-up of his or her tear film 1.This simple test involves the use of a slit-lamp, set on a bright light setting with a cobalt blue filter: 2.Instil fluorescein into the lower fornix. Ask the patient to blink several times and then stop. 3.Measure the time between the last blink and the first appearance of a dark spot on the cornea (formation of a dry area) on the otherwise continuously stained tear film. A tear break-up time of less than 10 seconds suggests a dry eye.
BUT YOU ARE TOUCHING THE FILM!!!!!!!!!!!!!!!!!!!

17. In the F B U T test the presence of fluorescein in the tears may stimulate reflex tearing and may also result in changes to the tear film properties. To overcome these potential limitations,

18. Non Invasive Break up time (NIBUT)
They are called “Non Invasive” because the eye is not touched. 1.Instruments such as a Keratometer, hand-held Keratoscope or Tearscope are required to measure NIBUT. 2.A pre-rupture phase that precedes actual break up of the tear film can also be observed with some techniques. This pre-rupture phase is termed Tear Thinning Time (TTT). Measurement is achieved by observing the distortion (TTT) and/or break up (NIBUT) of a keratometer mire (the reflected image of keratometer grid). The clinician focuses and views the crisp mires, and then records the time taken for the mire image to distort (TTT) and/or break up (NIBUT). 3.NIBUT measurements are longer than fluorescein break up time. NIBUT values of less than 15 seconds are consistent with dry eyes.

19. Techniques in measuring NBUT
INSTRUMENT
TARGET
BACKGROUND
ADDITIONAL
COMMENT
AUTHOR
KERATOMETER
MIRE
DARK FIELD
ONLY SHOWS REFLECTED DISTURBANCES 3MM AREA
PATEL
MODIFIED
HIR-CAL GRID
NORMAL USE OF KERATOMETER RESTRICTED
HIRJI
HANDHELD
KERATOSCOPE
LOVERIDGE
GRID
TEARSCOPE,
TOPO-ANALYZER
WHITE FIELD
VARIOUS COLOURS
CAN SHOW EVALUATION OF TEAR STRUCTURE
GUILLON
FEINBAUM

20. Tear Prism Height & Speed of filaments.

21. Routine
A clinical routine to incorporate the measurement of the tear film prism height: • Immediately below the pupil centre • 5mm nasally • 5mm temporally Try to avod fluorescein!

22. WATCH SPEED OF FILAMENTS IN TEAR FILM, RAPID MOVEMENT
TO WATERY, SLOW OR RUGGID MOVEMENT TOO OILY

23. TEAR VOLUME TESTS

24. Schirmer test 2.Prepare a filter paper (5mm x 35mm with folded end).
1.Instil a drop of local anaesthetic into the eye, (optional).
2.Prepare a filter paper (5mm x 35mm with folded end).
3.Gently dry the eye.
4.Apply the filter paper with the folded end hooked onto the lower lid margin at the junction between the middle and outer third (take care not to touch the cornea).
5.Tell patient to keep their eye open and blink normally.
6.Measure the amount of wetting after 5 minutes: 13-15mm wetting rules out a dry eye; 6-10mm is borderline and less than 6mm indicates dry eye.
7.The filter paper strips can cause reflex tearing and may require the use of anaesthetic agents. However, they still provide a measurable clinical indication of dry eye.

25. DO NOT USE!!! 1. USE OF ANAESTHETIC? 2. 5 MINUTES! 3. ACCURACY?
4. BOTHERSOME FOR PATIENT
DO NOT USE!!!

26. The LACRYMETER™ by ECCG GROUP Ltd

27. The Lacrymeter A paper strip impregnated with phenol red dye.
Phenol red is pH sensitive and changes from yellow to red when wetted by tears.
On the other end a sensitive pH dye is used.

28. Pretest
Examining the patient performing the LacrypHmeter™ test does not require any anaesthetic. If patient is using any kind of eye drop, the patient must not instill drops 1 hour before the examination. However, if the patient is wearing contact lenses, these can be worn during performance of test.

29. Instruction for use
1. Place the 3mm hook over the lower eyelid approximately 5mm from the lateral canthus when the eyelid is pulled out slightly, release the eyelid and start a stop clock and ask patient to look straight ahead and blink as normal. 2. Each eye is individually tested with open eyes and normal blinking for 30 seconds. 3. Remove the strip gently. Pull the lower eyelid down before removing the thread and patient will not have any discomfort. 4. Measure the entire length of where the strip has changed color from yellow to red including the fold.

30. Lacrymeter™ Functional Mode
Yellow (acidic) color on removal from wrapper and before contact with tears Changes to red (basic) indicating the volume of tears soaked up over the time of contact Borderline If change of color of thread less than 10mm, the tear quantity must be considered to be low. And the eye must be suspected to being dry. For pH. Compare with supplied scale of pH. Indicating acidic or basic tears.A pH meter provides a value as to how acidic or alkaline a liquid is. The basic principle of the pH meter is to measure the concentration of hydrogen ions. Acids dissolve in water forming positively charged hydrogen ions (H+).

31. The LacrypH© Test one eye at a time.
Gently touch eye temporally for 2-4 seconds with the pH end of strip
pH stands for power of hydrogen, which is a measurement of the hydrogen ion concentration in the body. The total pH scale ranges from 1 to 14, with 7 considered to be neutral. A pH less than 7 is said to be acidic and solutions with a pH greater than 7 are basic or alkaline.

32. More on pH
The mean pH values of 40 eyes from 20 healthy volunteers was 7.50 (SD ± 0.23), which corresponded well with those measured by the micro pH-meter. CONCLUSIONS. The method described was useful in measuring the pH of the precorneal tear film of humans with minimal invasion. Depleted mucous: Yes. Indirectly because the mucous level is down. Meibomian gland dysfuntion: Yes. Indirectly because the evaporation goes up

33. Treat pH
If tears too alcidic - give tear substitute that is basic in pH
If tears too basic - give tear substituted that is alcidic in pH.

34. STAIN TESTS

35. Rose Bengal Test DO NOT USE!!!!!!!!
The dye has been shown to be toxic to corneal epithelium at routine concentrations.
Stinging
DO NOT USE!!!!!!!!

36. Lissamine Green

37. Lissamine green staining like Rose Bengal, colours any desiccated and dying cells on the ocular surface. However, lissamine green may be better accepted by patients as it lacks the slight burning or stinging sensation typically found with rose Bengal.

39. HYPEROSMOLARITY TEST

40. Tearlab Osmolarity System
The disposable probe, touched onto the lower tear meniscus at the lid margin, collects a nanolitre sample of tears, which is analysed within seconds to provide the clinician with an osmolarity reading. Normal values lie around 304mOsm/kg while values over 320mOsm/kg indicate dry eye. (Mainly used in research).
TOO EXPENSIVE!
HOW LONG WILL IT BE AVAILABLE???