Presentation is loading. Please wait.

Presentation is loading. Please wait.

Determination of in vitro postantibiotic effects in Staphylococcus aureus and Escherichia coli by [3H]thymidine incorporation  Magnús Gottfredsson, Helga.

Similar presentations


Presentation on theme: "Determination of in vitro postantibiotic effects in Staphylococcus aureus and Escherichia coli by [3H]thymidine incorporation  Magnús Gottfredsson, Helga."— Presentation transcript:

1 Determination of in vitro postantibiotic effects in Staphylococcus aureus and Escherichia coli by [3H]thymidine incorporation  Magnús Gottfredsson, Helga Erlendsdóttir, Adalsteinn Gudmundsson, Sigurdur Gudmundsson  Clinical Microbiology and Infection  Volume 2, Issue 2, Pages (October 1996) DOI: /j tb00213.x Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions

2 Figure 1 Examples of growth (CFU/mL) and [3H] thymidine incorporation (counts/min) curves of E. coli ATCC Circles, unexposed control organisms; triangles, organisms during PAE after exposure to doxycyclin 4 x MIC for 1 h. Closed symbols, viability counts (log10(CFU/mL)); open symbols, [3H] thymidine incorporation (counts/min). In the example, the PAE determined by viable counts was 1.2 h and by [3H] thymidine incorporation 1.6 h. Clinical Microbiology and Infection 1996 2, DOI: ( /j tb00213.x) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions

3 Figure 2 Comparison of the [3H] thymidine incorporation method and viability counting for PAE determination in S. aureus. Bacterial counts post-drug removal were > 104 CFU/mL (range: 2.5 × 104 to 6 × 105 CFU/mL). Columns represent means from two to three experiments. Bars represent range. Di, dicloxacillin; Ge, gentamicin; Va, vancomycin; Rif, rifampin. a: data from one experiment. Clinical Microbiology and Infection 1996 2, DOI: ( /j tb00213.x) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions

4 Figure 3 Comparison of the [3H]thymidine incorporation method and viability counting for PAE determination in E. coli. Bacterial counts post-drug removal were >104 CFU/mL (range: 1.5 × 104 to 2.3 × 106 CFU/mL). Columns represent means from two to three experiments. Bars represent range. Ge, gentamicin; To, tobramycin; Dx, doxycycline; Rif, rifampin; Im, imipenem. Clinical Microbiology and Infection 1996 2, DOI: ( /j tb00213.x) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions

5 Figure 4 Correlation between CERT in S. aureus and E. coli as determined by viability counting and [3H] thymidine incorporation. Closed circles: E. coli; open circles: S. aureus. Clinical Microbiology and Infection 1996 2, DOI: ( /j tb00213.x) Copyright © 1996 European Society of Clinical Infectious Diseases Terms and Conditions


Download ppt "Determination of in vitro postantibiotic effects in Staphylococcus aureus and Escherichia coli by [3H]thymidine incorporation  Magnús Gottfredsson, Helga."

Similar presentations


Ads by Google