1. Virginia iGEM
Workshop #2
High School Education Series

2. Workshop Plan Virginia iGEM
Lesson: Genes, Backbones, and BioBrick Structure
Case Study: FtsZ and
Minicell Formation
(VGEM 2013)
Activity: DNA Extraction
Workshop #2
High School Education Series
Virginia iGEM

3. Genes Virginia iGEM Workshop #2 High School Education Series
Workshop #2
High School Education Series
Virginia iGEM

4. Promoters and Repressors
Genetic Toggle Switch
Repressilator
Workshop #2
High School Education Series
Virginia iGEM
For more information, see: Khalil, A. S., & Collins, J. J. (2010). Synthetic biology: Applications come of age. Nature Reviews Genetics, 11,

5. Gene Construct Structure
Ribosome Binding Site
Promoter
RBS
Gene
Terminator
Green Fluorescent Protein
Promoter
RBS
Gene
RBS
GFP
Terminator
(Reporter Gene)
Workshop #2
High School Education Series
Virginia iGEM

6. Fluorescence
for fun… 

7. for science…

8. Plasmids Virginia iGEM Workshop #2 High School Education Series
Electron Micrograph of Ruptured E. Coli Cell
Workshop #2
High School Education Series
Virginia iGEM

9. Plasmid Backbone Virginia iGEM GENE Workshop #2
High School Education Series
Virginia iGEM

10. Restriction Enzymes Virginia iGEM Workshop #2
Sticky Ends
Workshop #2
High School Education Series
Virginia iGEM

11. Case Study: FtsZ and Minicell Formation (VGEM 2013)
Z-Ring Off-Center
Workshop #2
High School Education Series
Virginia iGEM

12. Activity: DNA Extraction
Workshop #2
High School Education Series
Virginia iGEM

13. Any Questions? Virginia iGEM Workshop #2 High School Education Series
Workshop #2
High School Education Series
Virginia iGEM