Presentation is loading. Please wait.

Presentation is loading. Please wait.

Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice  Zaraq Khan, M.B.B.S., Heather.

Similar presentations


Presentation on theme: "Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice  Zaraq Khan, M.B.B.S., Heather."— Presentation transcript:

1 Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice  Zaraq Khan, M.B.B.S., Heather S. Wolff, M.D., Jolene R. Fredrickson, M.S., David L. Walker, M.S., Gaurang S. Daftary, M.D., Dean E. Morbeck, Ph.D.  Fertility and Sterility  Volume 99, Issue 3, Pages e2 (March 2013) DOI: /j.fertnstert Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 (A) Blastocyst rates of one-cell mouse embryos from different mouse embryos to increasing concentrations of cumene peroxide in mineral oil. *P<.05; **P<.01 vs. control. (B) LC50 values for cumene hydroperoxide in mineral oil for blastocyst development of one-cell mouse embryos from different strains of mice. Fertility and Sterility  , e2DOI: ( /j.fertnstert ) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Number of cells per blastocyst for embryos of different strains treated with cumene hydroperoxide in mineral oil. *P<.05 vs. control. Fertility and Sterility  , e2DOI: ( /j.fertnstert ) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

4 Figure 3 (A) Blastocyst rates of one-cell mouse embryos from different mouse embryos to increasing concentrations of Triton X-100 in culture media. *P<.05; **P<.01 vs. control. (B) LC50 values for Triton X-100 in culture media for blastocyst development of one-cell mouse embryos from different strains of mice. Fertility and Sterility  , e2DOI: ( /j.fertnstert ) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

5 Figure 4 Number of cells per blastocyst for embryos of different strains treated with Triton X-100 in culture media. *P<.05 vs. control. Fertility and Sterility  , e2DOI: ( /j.fertnstert ) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

6 Supplemental Figure 1 (A) Blastocyst rates of one-cell mouse embryos from different mouse embryos to increasing osmolality. *P<.05; **P<.01 vs. control. (B) LC50 values for osmolality of culture media for blastocyst development of one-cell mouse embryos from different strains of mice. Fertility and Sterility  , e2DOI: ( /j.fertnstert ) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

7 Supplemental Figure 2 Number of cells per blastocyst for embryos of different strains treated with increasing osmolality. *P<.05 vs. control. Fertility and Sterility  , e2DOI: ( /j.fertnstert ) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions


Download ppt "Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice  Zaraq Khan, M.B.B.S., Heather."

Similar presentations


Ads by Google