1. Dr. Kamlesh Palandurkar Department of Biochemistry IMS BHU
Presentation for the Design and Innovation Centre Designing & fabricating of a novel Bilirubin interference removal device for Clinical Laboratories
Dr. Kamlesh Palandurkar
MBBS,MD,DNB
Assistant Professor
Department of Biochemistry IMS BHU

2. The Problem
Bilirubin is one of the interfering factors in serum and plasma.
Many a diagnostic parameters like Serum creatinine, cholesterol, uric acid and many others tend to show deviated readings in presence of elevated levels of bilirubin i.e. Jaundice.

3. What we are planning to do??
To design and develop a devise that will intensify this process and will aid transformation of bilirubin so that this bilirubin interference problem can be solved within short period to aid diagnosis.
This will use two following principles
The black body effect
The laser

4. Treat the sample with these two things and take readings for analytes and bilirubin for the effectiveness of the technique

5. What are prospects of this design.
Petenting the design.
Publications
Marketing the product as a accessory of the auto analysers or as a independent small instrument which will aid the laboratories to overcome this problem.
Time Period
Period of this project will be 1.5 to 2 years and it will be completed by that time.

6. Justification for the budget
Sl. No.
Item
Quantity
Estimated cost
Justification 1
Equipments
Equipment Designing and fabricating.
Laser Light emitter and controller
150000/-
For Designing and fabricating
As Source of light
These parts are indispensible and integral part of the design. So their purchase is essential. 2
Contingency
Bilirubin standard
Reagents for different analyte Kits for Bilirubin, creatinine, cholesterol, urea, uric acid, glucose estimation.
120000/-
For Analysing the results of the equipments on the serum analytes 3
Consumables
Micropipette ,Micro Tips, Centrifuge tubes , Eppendorf tubes, Vaccutainers, Syringes, Cryoboxes, and chemicals etc.
80000/-
Accessories for research purpose
Total Rs5,00,000/-

7. Justification
Only indispensable equipment components are being quoted. The Whole Laser assembly costs more than 10 lakhs but I am quoting laser Component required for my experiment of interest.
Infrastructure of Department of Physics, BHU and Department of Biochemistry IMS BHU for laser related work will be utilized.
The assembly of the equipment and computer assisted designing will be done.

8. Justification:
Bilirubin standard and other chemicals are also expensive.
MP BIOMEDICALS
BILIRUBIN  from Porcine Gall Bladder Orange Yellow Powder
CAS Number 
Molecular Formula C33H36N4O6
Molecular Weight 584.7
Beilstein Registry Number 74376
EC Number
MDL Number MFCD
g- Rs 71,697.55

9. References
Blijenberg BG, Liesting EC, Zwang L. Creatinine and automatic analyzers in relation to icteric specimens. Eur J Cim Chem Biochem 1992;30:
Crocker H, Shephard MDS, White GH. Evaluation of an enzymatic method for determining creatinine in plasma. J ClinPathol 1988;41:
Spain MA, Wu AHB. Bilirubin interference with determinationof uric acid, cholesterol, and triglycerides in commercial peroxidase-coupled assays, and the effect of ferrocyanide. Cke Chem 1986;32:
Sonntag 0, Schumann G. Evalvierung der Ektachem-singleslide Methode zur Bestimmung der Creatinin-konzentration in Serum. Lab Med 1989;13:351-6.
Witte DL, Brown LF, Feld RD. Effects of bilirubin on detection of hydrogen peroxide by use of peroxidase. Cm Chem 1978;24: