1. AD AD E. coli J5 LPS (+) (-) 150 75 50 37 Gram-negative LPS 25
Figure e-1. Immunoabsorption specificity controls in AD brain. Western blot of AD brain using antibody against Gram-negative LPS (RayBiotech, MD ) as well as a blot after immunoprecipitating the antibody with E. coli LPS (VWR, , antibody:LPS = 1:10). This anti-Gram-negative LPS antibody detected a large band at 37kD (arrow, right panel) and a smaller band at 150kD, whereas E. coli LPS immuno-depleted antibody showed the 150kD band only with complete loss of the 37kD band (left panel). This is strong evidence the antibody detects LPS in the 37kD band, and this is the same molecular weight as 37kD large band seen in Fig. 1D with the Abcam anti-LPS antibody (lower panel). AD = Alzheimer’s disease.