1. Differential seminal plasma proteome according to semen retrieval in men with spinal cord injury 
Barbara Ferreira da Silva, M.Sc., Gustavo Henrique Martins Ferreira Souza, Ph.D., Edson Guimarães lo Turco, D.V.M., Ph.D., Paula Toni Del Giudice, M.Sc., Thiesa Butterby Soler, D.V.M., Ph.D., Deborah Montagnini Spaine, Ph.D., Milton Borrelli, M.D., Fábio César Gozzo, Ph.D., Eduardo Jorge Pilau, Ph.D., Jerusa Simone Garcia, Ph.D., Christina Ramires Ferreira, D.V.M., Ph.D., Marcos Nogueira Eberlin, Ph.D., Ricardo Pimenta Bertolla, D.V.M., Ph.D. 
Fertility and Sterility 
Volume 100, Issue 4, Pages e3 (October 2013)
DOI: /j.fertnstert
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Illustrative representation of highlighted clusters found in the interaction network of differentially secreted proteins from (A) control, (B) penile vibratory stimulation, and (C) electroejaculation groups. Color represents unique group protein (red), protein common to all groups (green), or protein common to two groups (blue), according only to the “shotgun proteomics” experiment (MS) results. Results from the two-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis followed by tandem MS were after uploaded and merged to these networks for final gene ontology analysis.
Fertility and Sterility  , e3DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

3. Supplemental Figure 1
Coomassie-stained two-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis maps of seminal plasma for (A) control, (B) penile vibratory stimulation, and (C) electroejaculation groups. Molecular mass separation is 225–12 kDa (top to bottom). Numbered spots indicate proteins that have statistically significant differential expression between groups according to the Imagemaster 2D Platinum 7.0 software (GE Healthcare), which made matches between the spots of different gels to show differences between individuals. One gel was chosen as a representative map of its group. The three groups were compared, and 39 spots of interest (matches) were manually excised from the gels, trypsin digested, and used for tandem mass spectrometry analysis. Identification results for these spots are shown in the Supplemental Tables.
Fertility and Sterility  , e3DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

4. Supplemental Figure 2
Some functional annotations for the gene ontology categories (A) “biologic process,” (B) “cellular component,” and (C) “molecular function” enriched by proteins exclusively identified in the seminal plasma of controls and spinal cord injury (SCI; penile vibratory stimulation and electroejaculation ) men.
Fertility and Sterility  , e3DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions