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Operation of a Purified DNA nanoactuator

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Presentation on theme: "Operation of a Purified DNA nanoactuator"— Presentation transcript:

1 Operation of a Purified DNA nanoactuator
F. C. Simmel and B. Yurke 7th International Meeting on DNA Based Computers Summerized by Lee, In-hee SNU Biointelligence Lab.

2 © 2001, SNU BioIntelligence Lab, http://bi.snu.ac.kr/
Introduction DNA nanoactuator Induce motion on a nanomolecuar scale By base-pairing interactions between DNA strands Have a tendency to form dimers or multimers during assembly Harmful effect on performance © 2001, SNU BioIntelligence Lab,

3 © 2001, SNU BioIntelligence Lab, http://bi.snu.ac.kr/
Operation Principle © 2001, SNU BioIntelligence Lab,

4 © 2001, SNU BioIntelligence Lab, http://bi.snu.ac.kr/
Operation Principle DNA nanoactuator as a thermodynamic engine Consume chemical energy, produce a waste product Monitored by FRET(Fluorescence Resonance Energy Transfer) © 2001, SNU BioIntelligence Lab,

5 Dimer Formation Not all strands properly assemble into nanoactuators
Fig. 2 Occur only during assembly Unstable © 2001, SNU BioIntelligence Lab,

6 Experiments and Results
Fig. 3 Fig. 4 © 2001, SNU BioIntelligence Lab,

7 Experiments and Results
Fig. 5 Fig. 6 © 2001, SNU BioIntelligence Lab,

8 © 2001, SNU BioIntelligence Lab, http://bi.snu.ac.kr/
Conclusion Correctly assembled devices can be purified © 2001, SNU BioIntelligence Lab,

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