1. A Motion Direction Preference Map in Monkey V4
Peichao Li, Shude Zhu, Ming Chen, Chao Han, Haoran Xu, Jiaming Hu, Yang Fang, Haidong D. Lu 
Neuron 
Volume 78, Issue 2, Pages (April 2013)
DOI: /j.neuron
Copyright © 2013 Elsevier Inc. Terms and Conditions

2. Figure 1 Functional Maps in V4
(A) A schematic of the macaque brain showing the imaging region (green circle) and sulcus locations. lu, lunate; st, superior temporal; ec, external calcarine sulcus; io, inferior occipital sulcus; ot, occipitotemporal.
(B) In vivo image of the blood vessel pattern of the imaging region, ∼20 mm diameter. A, anterior; L, lateral. Scale bar, 2 mm.
(C–I) Functional maps (t-maps) obtained with optical imaging. Icons below each map indicate stimulus conditions and statistical comparisons (paired t test, two tailed). Dotted lines indicated area boundaries between V1, V2, and V4. (C) shows an ocular dominance map showing ocular dominance columns in V1. (D) shows a color preference map obtained by comparing isoluminant red-green and achromatic gratings. Red arrows point to color-preferring domains in V4. Short red bars on V2 borders indicate approximate thin stripe locations that are preferentially activated by color stimuli. The same red bars are transferred to maps (E)–(H). (E) and (F) show a 0°-versus-90° orientation preference map (E) and a 45°-versus-135° orientation preference map (F). Color preference regions in V4 (red arrows) and V2 (red bars) have weak orientation selectivity (gray pixels). (G) and (H) show a down-versus-up direction preference map (G) and a right-versus-left direction preference map (H). Yellow dashed ovals outline clusters of direction-preferring domains in V4 (small black and white patches). Yellow arrowhead indicates a direction preference map in V2. (I) shows a condition-randomized map obtained by randomly swapping in half of the rightward motion and leftward motion trials; same data set as in (H). No significant domains are observed in this map. Scale bar in (B) also applies to (C)–(I).
See Figures S1, S2, and S3.
Neuron  , DOI: ( /j.neuron )
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3. Figure 2 Close-Up Views of Direction-Preferring Domains in V2 and V4
(A–C) Responses from three direction-preferring regions to eight different directions in Case 1. (A) shows a V2 direction-preferring region (yellow arrowhead in Figures 1G and 1H); (B) and (C) show two V4 direction-preferring regions (bottom left and top left ovals in Figures 1G and 1H). Each small panel is the difference between the direction indicated by the corresponding arrow and its opposite direction. Yellow circles in (B) mark a singular downward-preferring domain in V4, which does not have any neighboring domains. This type of singular domain is often observed in V4 but not so common in V2.
(D–F) Vectorized summations of the eight small panels in (A) through (C). Each direction is coded in a specific color; the color index is shown under (D). Note that, in (A), locations of the V2 activation zones shift as stimulus direction shifts, forming a linear shifting region, indicated with a white bracket in (D). In contrast, direction-preferring domains in V4, shown in (B) and (C), often appear as isolated singular domains. Their polar maps, shown in (E) and (F), also have few linear patterns; nevertheless, representation for every direction (every color) can be found in these direction preference maps. Scale bars, 1 mm.
Neuron  , DOI: ( /j.neuron )
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4. Figure 3 Functional Maps from Cases 2–4
One case is shown per row. All three cases are taken from the left hemisphere (illustrated in inset at top left corner). The four columns are as follows: ocular dominance maps (first column), color preference maps (second column), orientation preference maps (third column), and direction preference maps (fourth column). Maps were obtained using the same stimuli as those used in Case 1. Generally, these maps have similar features to those observed in Case 1. In V4, patches of color-, orientation- and direction-preferring domains appear to occupy restricted regions. From case to case, a large variation of V4 domain locations was observed, unlike a uniform distribution in V1 or a periodical distribution in V2. Nevertheless, direction-preferring domains were observed in V4 in every case. Plot conventions are the same as in Figure 1. A, anterior; L, lateral. Scale bars, 2 mm; different cases (rows) show different scale bars.
Neuron  , DOI: ( /j.neuron )
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5. Figure 4
Chamber Photos and Functional Maps of the Same Cortical Region across Several Days
Images in the three rows were collected on three different days. The first column shows photos of the imaging chamber taken by a digital camera, and the second column shows blood vessel images obtained with the imaging camera under 546 nm green light illumination; their labels in the first row indicate major sulci (lu, lunate; st, superior temporal; io, inferior occipital sulcus) and visual areas (V1, V2, and V4). The third column shows orientation preference maps (horizontal versus vertical gratings). Dotted lines indicate area boundaries between V1, V2, and V4. The fourth column shows direction preference maps (upward versus downward directions), from which the framed regions are expanded in the fifth column. These maps show that V4 direction-preferring domains, like orientation-preferring domains, can be repeatedly imaged from the same locations on different days. Functional maps from different days were aligned based on blood vessel maps. Scale bars in the second and fifth columns, 2 mm.
See Figure S4.
Neuron  , DOI: ( /j.neuron )
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6. Figure 5
Clustering of Direction-Selective Neurons in V4 Direction-Preferring Domains
(A) Color-coded direction polar map illustrates the locations of four recoding sites (sites 1–3: direction-preferring domains, site 4: non-direction-preferring domain).
(B) Blood vessel image of the same cortical region used to guide electrode penetrations. Scale bar, 1 mm; this also applies to (A).
(C) Direction selectivity of 19 neurons recorded from four penetrations. The imaged polar maps on the left indicate the direction selectivity (sites 1–3, colored arrows) or the orientation selectivity (site 4, bars) at the recording site. Direction polar plots on the right show the direction selectivity of single neurons recorded from the same penetration at different depths (see label at top of each plot). In each polar plot, response spike rates (black dots) are fitted with a von Mises function (black curve), and the gray region around the tuning function represents 1 SEM. Most neurons recorded in direction-preferring domains showed strong direction selectivity, and their preferred direction was consistent with the optical imaging results. Neurons recorded from non-direction-preferring domains showed weak or no direction selectivity.
(D) Distributions of direction selectivity index values (DI = 1 − Rn/Rp) for all neurons collected in direction-preferring domains (black bars) and in non-direction-preferring domains (gray bars). Neurons in direction-preferring domains showed much higher direction selectivity than those in non-direction-preferring domains (p = 1.01 × 10−6, Kolmogorov-Smirnov test).
(E) Distribution of orientation selectivity index values for the same neurons collected in direction-preferring domains (black bars) and in non-direction-preferring domains (gray bars). These two groups of neurons do not differ in orientation selectivity (p = 0.48, Kolmogorov-Smirnov test).
See Figure S5.
Neuron  , DOI: ( /j.neuron )
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7. Figure 6 Overlap between V4 Functional Maps
(A–C) Orientation- (A), color- (B), and direction (C)-preferring maps from Case 1, same as Figures 1E, 1D, and 1G, respectively. Domain masks are overlaid on to the representative maps with different colors (orange, orientation mask; pink, color mask; green, direction mask). Scale bar, 2 mm; this applies to (A) through (F).
(D) Top shows spatial overlaps between direction (green) and orientation (orange) maps revealing significant overlapping pixels (yellow). Bottom shows the percentage of direction pixels overlapping with the orientation mask (yellow bar) is significantly larger than the percentage of orientation pixels in total V4 areas (orange bar) (p = 0.002, two-tailed t test, n = 7), which indicates a tendency of direction and orientation preference maps to overlap.
(E) Similarly to (D), comparison of direction and color masks also indicates a tendency for direction and color preference maps to overlap in V4 (p = 0.023, two-tailed t test, n = 7).
(F) Color and orientation do not show a tendency to overlap (p = 0.20, two-tailed t test, n = 7). Error bar, 1 SEM.
See Figure S6.
Neuron  , DOI: ( /j.neuron )
Copyright © 2013 Elsevier Inc. Terms and Conditions