1. Mean and difference Raman spectra of T cells isolated from spleen of sham mice and endotoxemic mice for time points 1, 4, 10, and 30 d.
Mean and difference Raman spectra of T cells isolated from spleen of sham mice and endotoxemic mice for time points 1, 4, 10, and 30 d. Top row: mean Raman spectra of (A) CD4+CD8+ T cells, (B) CD4+ T cells, and (C) CD8+ T cells isolated from spleen of sham C57BL/6 mice and LPS-insulted C57BL/6 mice (time point of LPS insult is shown on right axis). Bottom row: difference Raman spectra between (D) CD4+CD8+ T cells, (E) CD4+ T cells, and (F) CD8+ T cells from sham mice (spectra in black) and LPS-insulted mice (spectra in red). Negative peaks belong to LPS-treated cells, and positive peaks belong to healthy cells. The spectra are shifted in y-axis for clarity. For each time point, pooled spleen obtained from 9 to 12 mice (both male and female) per condition have been used (see Supplemental Table IA). A minimum of three biological repetition and Raman spectroscopic investigations were done for each time point (see Supplemental Table IA). T cells were isolated, and CD4+ T cells and CD8+ T cells were purified using automated negative magnetic-assisted cell sorting using standard isolation kit.
Anuradha Ramoji et al. ImmunoHorizons 2019;3:45-60
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