1. Phosphatase

3. ‘Experiments on product inhibition with phosphatase’
Higher Biology
Metabolism and Survival:
1. Metabolic pathways and their
control
- Control of metabolic pathways
‘Experiments on product inhibition with phosphatase’

4. ‘Experiments on product inhibition with phosphatase’
Higher Human Biology
Human Cells:
6. Metabolic pathways
- Control of metabolic pathways
‘Experiments on product inhibition with phosphatase’

5. Phosphatase
Phosphatase enzymes from plants: a versatile resource for post-16 courses
Meatyard, B. (1999),
J. Biol. Educ., 33(2),

6. The Assay

7. PP = phenolphthalein (product)
PPP = phenolphthalein phosphate (substrate)

8. Preparing the enzyme extract (Part A)
Running the assay (Part B)

10. Creating the extract

11. Germination

12. Maceration
Distilled water

13. Loading centrifuge tube

14. Centrifugation

15. Retain supernatant

16. Running the Assay

17. Running the assay

19. Investigations Effect of temperature Effect of pH
Phosphatase levels from different species
Phosphatase levels in different locations
Effect of phosphate concentration

20. Results

21. Results