1. Volume 119, Issue 1, Pages 151-161 (July 2000)
Effects of dietary folate on DNA strand breaks within mutation-prone exons of the p53 gene in rat colon 
Young–In Kim, Shaila Shirwadkar, Sang–Woon Choi, Martina Puchyr, Yang Wang, Joel B. Mason 
Gastroenterology 
Volume 119, Issue 1, Pages (July 2000)
DOI: /gast
Copyright © 2000 American Gastroenterological Association Terms and Conditions

2. Fig. 1
(A) Mean plasma folate concentrations in 3 groups of rats fed different levels of dietary folate. Different letters at each time point indicate statistically significant differences (P < 0.001). (B) Mean colonic mucosal folate concentrations in 3 groups of rats fed different levels of dietary folate. Different letters at each time point indicate statistically significant differences (P < 0.05). (C) Mean plasma homocysteine concentrations, an inverse indicator of folate status, in 3 groups of rats fed different levels of dietary folate. Different letters at each time point indicate statistically significant differences (P < 0.01).
Gastroenterology  , DOI: ( /gast )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

3. Fig. 2
Effect of dietary folate deficiency and supplementation on DNA strand breaks within exons 5-8 of the p53 gene as assessed by qPCR. In this assay, the decrease in the quantitative recovery of 32P-labeled PCR amplification product is proportional to the extent of DNA lesions present within these exons. Results are expressed as the extent of [32P]qPCR product recovery, defined as a percentage of the control. Different letters at each time point indicate statistically significant differences (P < 0.05). The recovery of [32P]qPCR product in the folate-deficient group decreased progressively over the study period (P < 0.02, linear trend), indicating progressive accumulation of DNA strand breaks within these exons with extended folate deprivation.
Gastroenterology  , DOI: ( /gast )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

4. Fig. 3
Effect of dietary folate deficiency and supplementation on DNA strand breaks within exons 2-4 of the p53 gene as assessed by qPCR. Results are expressed as the extent of [32P]qPCR product recovery, defined as a percentage of the control. Different letters at each time point indicate statistically significant differences (P < 0.05).
Gastroenterology  , DOI: ( /gast )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

5. Fig. 4
(A) A representative autoradiogram of steady-state levels of p53 transcript among 3 groups of rats fed different levels of dietary folate at week 5. A 277-bp region in exons 6-7 of the rat p53 gene was amplified by RT-PCR from total RNA of the colonic mucosa. The amplified RT-PCR product from each sample was separated on an agarose gel, transferred to a nylon membrane using Southern blot, and hybridized with an internal oligonucleotide probe. (B) Effect of dietary folate deficiency and supplementation on steady-state levels of p53 transcripts as assessed by semiquantitative RT-PCR. Results are expressed as the percentage change in p53/GAPDH transcript ratios from control. Different letters at each time point indicate statistically significant differences (P < 0.04). Steady-state levels of p53 transcript progressively decreased in rats fed a folate-deficient diet, whereas they progressively increased in rats fed a supplemented diet during the study period (P < 0.05, linear trend).
Gastroenterology  , DOI: ( /gast )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

6. Fig. 4
(A) A representative autoradiogram of steady-state levels of p53 transcript among 3 groups of rats fed different levels of dietary folate at week 5. A 277-bp region in exons 6-7 of the rat p53 gene was amplified by RT-PCR from total RNA of the colonic mucosa. The amplified RT-PCR product from each sample was separated on an agarose gel, transferred to a nylon membrane using Southern blot, and hybridized with an internal oligonucleotide probe. (B) Effect of dietary folate deficiency and supplementation on steady-state levels of p53 transcripts as assessed by semiquantitative RT-PCR. Results are expressed as the percentage change in p53/GAPDH transcript ratios from control. Different letters at each time point indicate statistically significant differences (P < 0.04). Steady-state levels of p53 transcript progressively decreased in rats fed a folate-deficient diet, whereas they progressively increased in rats fed a supplemented diet during the study period (P < 0.05, linear trend).
Gastroenterology  , DOI: ( /gast )
Copyright © 2000 American Gastroenterological Association Terms and Conditions